Impact of SV40 T antigen on two multiple fission microalgae species Scenedesmus quadricauda and Chlorella vulgaris
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- 발행기관
- 국제인공지능학회(구 한국인터넷방송통신학회) 바로가기
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- 간행물
- The International Journal of Advanced Smart Convergence KCI 등재 바로가기
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- 통권
- Volume 7 Number 1 (2018.03)바로가기
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- 페이지
- pp.48-63
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A326314
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- The combination of Simian Virus40 (SV40)’s large T antigen with its replication origin is commonly used in molecular studies to enhance the expression of heterogeneous genes through multiplying the plasmid copy number. There are no reports related to the impact of the SV40 T antigen on plant, multiple fissional, cell-type. This study explores the response of two multiple-fission microalgal cells, Scenedesmus quadricauda and Chlorella vulgaris, to the expression of the T-antigen, with aim of applying SV40 T-antigen to increase the expression efficiency of foreign genes in the two species. Different levels of low-expression have been constructed to control the expression of SV40 T antigen using three heterogenous promoters (NOS, CaMV35S, and CMV). Chlorella cultures showed slowdown in the growth rate for samples harboring the T antigen under the control of CaMV35S and CMV promoters, unlike Scenedesmus cultures which showed no significant difference between samples and could have silenced the expression.
목차
Abstract
1. Introduction
2. Experiments
2.1 Isolation, purification and cultivation of Scenedesmus quadricauda and Chlorella vulgaris
2.2 Cassette construction
2.3 Vector construction
2.4 CaMV35S promoter activity
2.5 Electroporation and Cultivation
2.6 Modified protocol for screening the mitotic-division
2.7 DNA extraction, Quantitative and normal PCR
2.8 Immunofluorescence and Flow cytometry
2.9 Total RNA extraction, RT-PCR, and Transcription analysis by PCR
3. Results and discussion
3.1 Sequencing result
3.2 Polymerase chain reaction and Quantitative real time PCR
3.3 Flow cytometry analysis for Scenedesmus cultures
3.4 RT-PCR and Transcription analysis
3.5 Confirming the activity of the CaMV35S Promoter in Scenedesmus sp.
4. Conclusion
Acknowledgment
References
키워드
Scenedesmus quadricauda Chlorella vulgaris multiple fission SV40 T antigen Retinoblastoma(RB)-family저자
간행물 정보
발행기관
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- 발행기관명
- 국제인공지능학회(구 한국인터넷방송통신학회) [The International Association for Artificial Intelligence]
- 설립연도
- 2000
- 분야
- 공학>전자/정보통신공학
- 소개
- 인터넷방송, 인터넷 TV , 방송 통신 네트워크 및 관련 분야에 대한 국내는 물론 국제적인 학술, 기술의 진흥발전에 공헌하고 지식 정보화 사회에 기여하고자 한다.
간행물
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- 간행물명
- The International Journal of Advanced Smart Convergence
- 간기
- 계간
- pISSN
- 2288-2847
- eISSN
- 2288-2855
- 수록기간
- 2012~2025
- 십진분류
- KDC 326 DDC 380
이 권호 내 다른 논문 / The International Journal of Advanced Smart Convergence Volume 7 Number 1
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