Earticle

초록

영어

With the problem of cancer on the rise, a need to explore alternative cures such as nanoparticles (NPs) exists. The usual production of these NPs is costly and hazardous, which highlights the need for much eco-friendly and cost-efficient techniques. In this study, silver (Ag) NPs were synthesized using green synthesis method from the fruit extract of Morinda citrifolia. Fourier Transform Infrared (FT-IR) Spectroscopy was used to identify the functional groups. Morphological assessment of the synthesized NPs was done using Scanning Electron Microscopy (SEM). Determination of the elemental composition was done using Energy Dispersive X-ray (EDX). The effect of the synthesized NPs on MCF-7 human breast cancer cell line was evaluated using MTT Cytotoxicity Assay. SEM images revealed nanospherical particles with sizes ranging from 28 nm to 136 nm. EDX spectroscopy evaluations suggested the successful formation of NPs with purity of 32.20%. FT-IR showed a number of peaks that represent different functional groups of biological origin. The green synthesized Ag NPs exhibited cytotoxicity against human breast cancer cell (MCF-7) with inhibitory concentration (IC50) greater than 100 μg/mL. Data indicated that the green synthesized NPs possessed anti-cancer activity. No significant differences were revealed among the anticancer activities of the silver nanoparticles and among the anticancer activities of the positive control, Doxorubicin®, in all respective concentrations. The green synthesized Ag NPs were potential alternative cures for cancer.

목차

ABSTRACT
 1. INTRODUCTION
 2. MATERIALS AND METHODS
  2.1. Preparation of Fruit Extract
  2.2. Green Synthesis of Silver Nanoparticles
  2.3. Characterization of Synthesized Silver Nanoparticles
  2.4. MTT Cytotoxicity Assay
  2.5. Statistical Analysis
 3. RESULTS AND DISCUSSION
  3.1. Morphological Properties of Ag Nanoparticles
  3.2. Characterization of Nanoparticles
  3.3. MTT Cytotoxicity Assay Results
  3.4. Statistical Analysis
 4. CONCLUSION AND RECOMMENDATIONS
 5. ACKNOWLEDGEMENTS
 REFERENCES

키워드

저자

• Reisha Claffel Z. Ferraren [ Philippine Science High School – Central Visayas Campus, Talaytay, Argao, Cebu, Philippines ] Corresponding author
• Peter Gabriel A. Muaña [ Philippine Science High School – Central Visayas Campus, Talaytay, Argao, Cebu, Philippines ]
• Benito A. Baje [ Philippine Science High School – Central Visayas Campus, Talaytay, Argao, Cebu, Philippines ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  APEC Youth Scientist Journal [APEC 청소년 과학학술지]

• 간기

  반년간

• pISSN

  2005-5625

• 수록기간

  2009~2022

• 십진분류

  KDC 405 DDC 505