반응표면 분석법에 의한 표고균사체발효 적하수오 열수 추출조건의 최적화
Optimal hot water extraction conditions of fermented Polygonum multiflorum root by Lentinula edodes pegler mycelials using response surface methodology
This study was performed to determine optimal extraction conditions of fermented Polygonum multiflorum root by Lentinula edodes (JMI10079) Pegler mycelials using response surface methodology. The independent factors were extraction temperature (X1: 40–100oC), extraction time (X2: 2–10 hrs.), and the ratio of water to sample (X3: 33–100 mg/mL). Their effects were assessed on dependent variables of the extract properties, which included soluble solid contents (Y1), °Brix of sample extract (Y2), total polyphenol content (Y3), total flavonoid content (Y4), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical cation scavenging activity (Y5) and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (Y6). The experimental data obtained were fit to a second-order polynomial equation. The optimal extraction conditions for fermented P. multiflorum root were: X1: 91.22°C, X2: 7.72 hrs, and X3: 39.71 mg/mL.
목차
ABSTRACT 서론 재료 및 방법 재료 시약 반응표면 분석 시험 설계 고형분 함량 당도 측정 Total polyphenol 함량 측정 Total flavonoid 함량 측정 ABTS cation radical scavenging activity DPPH radical scavenging activity 통계분석 결과 및 고찰 추출물의 특성 고형분 함량 당도 측정 Total polyphenol 함량 및 Total flavonoid 함량 항산화 활성 열수 추출조건의 최적화 요약 감사의 글 References