Cha Ok Yim, Seung Min Lee, Hye Rim Kim, Anower Jabed, Chin Bum Lee, Byung Ki Kim
언어
영어(ENG)
URL
https://www.earticle.net/Article/A32268
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초록
영어
The aim of this study was to investigate whether addition of porcine epididymal fluid (pEF) into culture medium during in vitro maturation influences the nuclear maturation of porcine germinal vesicle (GV) oocytes. Porcine cumulus-oocyte complexes (COCs) from follicles were cultured in tissue culture medium 199 (TCM 199) containing pEF. After 48 hr of culture, oocytes were examined for evidence of GV breakdown, metaphase I, anaphase-telophase I, and metaphase II. The proportion of oocytes reaching at metaphase II (M II) stage was significantly (p<0.05) increased in oocytes cultured in the media supplemented with 10% pEF during in vitro maturation than in those without pEF regardless of cumulus presence or absence (54.6% vs 22.5%,51.7% vs 24.2%). The supplementation of pEF during maturation of oocyte enhanced oocytes maturation in a dose-dependent manner in vitro. Also significant differences (p<0.05) in the percentage of MII oocytes were observed according to exposure period in pEF. Present study suggests that pEF contains a enhancing component(s) for nuclear maturation of porcine immature oocytes in vitro.
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES
키워드
PorcineEpididymal fluidIn vitro maturationOocytes
저자
Cha Ok Yim [ Department of Molecular Biology, Dong-Eui University, Busan 614-714, Korea ]
Seung Min Lee [ Department of Molecular Biology, Dong-Eui University, Busan 614-714, Korea ]
Hye Rim Kim [ Department of Molecular Biology, Dong-Eui University, Busan 614-714, Korea ]
Anower Jabed [ Department of Molecular Biology, Dong-Eui University, Busan 614-714, Korea ]
Chin Bum Lee [ Department of Molecular Biology, Dong-Eui University, Busan 614-714, Korea ]
Byung Ki Kim [ Department of Molecular Biology, Dong-Eui University, Busan 614-714, Korea, Corresponding author. ]