Earticle

초록

영어

In order to generate transgenic goats expressing human growth hormone (hGH) in their mammary glands, goat β-casein/hGH hybrid gene was introduced into goat zygotes by pronuclear microinjection. DNA-injected embryos were transferred to the oviduct of recipients at 2-cell stage or to the uterus at morula/blastocyst stage after cultivation in glutathione-supplemented mSOF medium in vitro. Pregnancy and survival rate were not significantly different between 2-cell embryos and morula/blastocysts transferred to oviduct and uterus, respectively. One transgenic female goat was generated from 153 embryos survived from DNA injection. Southern blot analysis revealed that the transgenic goat harbored single-copy transgene with a partial deletion in its sequences. Despite of the partial sequence deletion, the transgene was successfully expressed hGH at the level of 72.1±15.1 μg/ml in milk throughout lactation period, suggesting that the sequence deletion had occurred in non-essential part of the transgene for the transgene expression. Unfortunately, however, the transgene was not transmitted to her offspring during three successive breeding seasons. These results demonstrated that goat β-casein/hGH gene was integrated into the transgenic goat genome in a mosaic fashion with a partial sequence deletion, which could result in a low level expression of hGH and a failure of transgene transmission.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

키워드

저자

• Chul-Sang Lee [ Department of Biological Science, Kunsan National University, Kunsan 573-701 ]
• Doo Soo Lee [ Hanmi Research Center, Hwaseong 445-813, Korea Research Institute of Bioscience and Biotechnology, Daejon 305-600 ]
• Nan-Zhu Fang [ Korea Research Institute of Bioscience and Biotechnology, Daejon 305-600 ]
• Keon Bong Oh [ Korea Research Institute of Bioscience and Biotechnology, Daejon 305-600 ]
• Sang-Tae Shin [ College of Veterinary Medicine, Chungnam National University, Daejon 305-764 ]
• Kyung-Kwang Lee [ Korea Research Institute of Bioscience and Biotechnology, Daejon 305-600, Corresponding authors ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636