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Comparison of Different Vehicles on Human Embryonic Stem Cells using Vitrification

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 30 No 4 (2006.12)바로가기
  • 페이지
    pp.279-285
  • 저자
    Jae Ho Lee, Gi Jin Kim, Sinae Kim, Won Woo Lee, Hey Jin Lee, Dong Ryul Lee, Hyung Min Chumg
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A32257

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원문정보

초록

영어
Vitrification has been suggested to be an effective method for the cryopreservation of human ES cells. However, the efficiency of vitrification with different vehicles remains a matter of ongoing controversy. The objective of this study was to assess the efficiency of cryopreservation in human ES cells by vitrification using different vehicles. A human ES cell line and a variety of vehicles, including microdroplet (MD), openpulled straw (OPS) and electron microscopic grid (EMgrid), were employed in an attempt to assess vitrification efficiency. In order to evaluate the survivability and the undifferentiated state of the postvitrified human ES cells, we conducted alkaline phosphatase staining and characterization via both RTPCR and immunofluorescence assays. The survival rates of the postvitrified human ES cells using MD, OPS and EMgrid were determined to be 61.5%, 66.6% and 53.8%, respectively. There also exist significant differences between slowfreezing and vitrification (p<0.01). However, no significant differences were detected between the vehicle types. Finally, the pluripotency of human ES cells after thawing was verified by teratoma formation. Cryopreservation using vitrification is more effective than slowfreezing, and the efficiency of vehicles proved effective with regard to the preservation of human ES cells.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

키워드

Human embryonic stem cells Miavdroplet Vitrification Cryopreservation

저자

  • Jae Ho Lee [ Stem Cell Research Laboratory, CHA Stem Cell Institute, Pochon CHA University, Seoul 135-081, Korea, Stem Cell Transplantation Center, CHA Biotech Co. Ltd., Seoul 135-081. Korea ]
  • Gi Jin Kim [ Stem Cell Research Laboratory, CHA Stem Cell Institute, Pochon CHA University, Seoul 135-081, Korea ]
  • Sinae Kim [ Stem Cell Research Laboratory, CHA Stem Cell Institute, Pochon CHA University, Seoul 135-081, Korea ]
  • Won Woo Lee [ Stem Cell Research Laboratory, CHA Stem Cell Institute, Pochon CHA University, Seoul 135-081, Korea, Stem Cell Transplantation Center, CHA Biotech Co. Ltd., Seoul 135-081. Korea ]
  • Hey Jin Lee [ Stem Cell Research Laboratory, CHA Stem Cell Institute, Pochon CHA University, Seoul 135-081, Korea, Stem Cell Transplantation Center, CHA Biotech Co. Ltd., Seoul 135-081. Korea ]
  • Dong Ryul Lee [ Stem Cell Research Laboratory, CHA Stem Cell Institute, Pochon CHA University, Seoul 135-081, Korea ]
  • Hyung Min Chumg [ Stem Cell Research Laboratory, CHA Stem Cell Institute, Pochon CHA University, Seoul 135-081, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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