Earticle

초록

영어

There are replete numbers of reports which have apparently shown that established patterns of methylation are critical for normal mammalian development. Here, we report expression of the DNA methyltransferases (Dnmts) family during mouse early development. Transcription of Dnmt1o occurs in one-cell and morula stage embryos, whereas Dnmt1s transcripts were detectable in all cells and tissues examined during the study. Dnmt3a1 transcript was detected in all cells and Dnmt3a2 transcript was particularly detected in the oocyte and 1-cell stages. Low level Dnmt3b1 transcripts were expressed ubiquitously in oocyte, 1-cell, and preimplantation embryos except 2～4 cell stages. Dnmt3b3 transcripts were only detected in E7.5 embryo and ovary. Furthermore, Dnmt3l transcripts were detectable in all cells and tissues examined. Unlike Dnmt1, both Dnmt3a and Dnmt3b proteins existed in the nucleus of preimplantation embryos till the morula stage. These Results suggest that differences Dnmts expression level exist and genomic DNA methylation patterns may be determined partly through differential expression of Dnmts during early development.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

키워드

저자

• Yeoung-Gyu KO [ AnimalB iotechnology Division, National Livestock Research Institute, RDA Suwon 441-706, Korea, Corresponding author ]
• Jong-Mu Kim [ AnimalB iotechnology Division, National Livestock Research Institute, RDA Suwon 441-706, Korea, Department of Animal Science, Chungbuk National University, Cheongiu 361-763, Korea ]
• Gi-Sun lm [ AnimalB iotechnology Division, National Livestock Research Institute, RDA Suwon 441-706, Korea ]
• Byoung-Chul Yang [ AnimalB iotechnology Division, National Livestock Research Institute, RDA Suwon 441-706, Korea ]
• Hwi-Cheul Lee [ AnimalB iotechnology Division, National Livestock Research Institute, RDA Suwon 441-706, Korea ]
• Hwan-Hoo Seong [ AnimalB iotechnology Division, National Livestock Research Institute, RDA Suwon 441-706, Korea ]
• Boh-Suk Yang [ AnimalB iotechnology Division, National Livestock Research Institute, RDA Suwon 441-706, Korea ]
• Hak-Jae chung [ AnimalB iotechnology Division, National Livestock Research Institute, RDA Suwon 441-706, Korea, Corresponding author ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636