Earticle

초록

영어

We have generated transgenic mice that expressed mouse extracellular superoxide dismutase (EC-SOD) in their skin. In particular, the expression plasmid DNA containing human keratin K14 promoter was used to direct the keratinocyte-specific transcription of the transgene. To compare intron-dependent and intron-independent gene expression, we constructed two vectors. The vector B, which contains the rabbit -globin intron 2, was not effective for mouse EC-SOD overexpression. The EC-SOD transcript was detected in the skin, as determined by Northern blot analysis. Furthermore, EC-SOD protein was detected in the skin tissue, as demonstrated by Western blot analysis. To evaluate the expression levels of EC-SOD in various tissues, we purified EC-SOD from the skin, lungs, brain, kidneys, livers, and spleen of transgenic mice and measured its activities. EC-SOD activities in the transgenic mice skin were approximately 7 fold higher than in wild-type mice. These results suggest that the mouse overexpressing vector not only induces keratinocyte-specific expression of EC-SOD, but also expresses successfully functional EC-SOD. Thus, these transgenic mice appeared to be useful for the expression of the EC-SOD gene and subsequent analysis of various skin changes, such as erythema, inflamation, photoaging, and skin tumors.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

저자

• Sung Hyun Kim [ School of Life Science and Biotechnology, Kyungpook National Universify, Daegu 702-701, Korea, Department of Animal Science and Biotechnology, Konkuk University, Seoul 143-701, Korea ]
• Myoung Ok Kim [ School of Life Science and Biotechnology, Kyungpook National Universify, Daegu 702-701, Korea ]
• Sang Gyu Lee [ School of Life Science and Biotechnology, Kyungpook National Universify, Daegu 702-701, Korea ]
• Zae Young Ryoo [ School of Life Science and Biotechnology, Kyungpook National Universify, Daegu 702-701, Korea, Department of Animal Science and Biotechnology, Konkuk University, Seoul 143-701, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636