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Uptake of Mitochondria1 DNA Fragment into Boar Spermatoza for Sperm-Mediated Gene Transfer

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 30 No 3 (2006.09)바로가기
  • 페이지
    pp.189-194
  • 저자
    Tae-Shin Kim, Cao Yang, Hee-Tae cheong, Boo-Keun Yang, Sang-Young Lee, Choon-Keun park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A31813

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원문정보

초록

영어
Sperm-mediated gene transfer (SMGT) can be used to transfer exogenous DNA into the oocyte at fertilization. The main objective of this study was to assess efficiency of transferring mitochondrial DNA (mtDNA) fragment into boar spermatozoa in either presence or absence of liposome and quality of transfected spermatozoa. The mtDNA of chicken liver was isolated and purified by phenol and alkaline lysis extraction, and it was inserted to plasmid. The genome of transfected spermatozoa treated with DNase Ⅰ was purified by alkaline lysis, and then amplified by the PCR analysis. After electrophoresis, DNA quantitation of each well was calculated by comparison of the band intensity with standard. As a result, exogenous DNA was composed of mtDNA fragment (1.2 kb) and plasmid (2.7 kb). On the other hand, efficiency of transfection by liposome (9.0±0.34 ng/l) in SMGT was higher than that by DNA solution (6.9±0.53 ng/l). However, there was no significant difference. Transfering exogenous DNA into spermatozoa was completed within 90 min of incubation. In another experiment, there were significant (p<0.05) differences between transfected spermatozoa using both DNA solution and DNA/liposome complexes with untreated spermatozoa for viability (70.8±1.80 and 68.0±2.16% vs. 83.3±1.69%, respectively) and motility (78.7±1.59 and 79.3±2.14% vs. 86.7±1.59%, respectively). This study indicates that exogenous mtDNA can be efficiently transferred into boar spermatozoa regardless of the presence of liposome, and transfected spermatozoa can also use insemination and in vitro fertilization to generate transgenic pig.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

DNA uptake SMGT Tiposome Transfection Boar spermatozoa

저자

  • Tae-Shin Kim [ College of Animal Life Sciences ]
  • Cao Yang [ College of Animal Life Sciences, college of Bioengineering Dalian University ]
  • Hee-Tae cheong [ School of Veterinaiy Medicine Kangwon National University ]
  • Boo-Keun Yang [ College of Animal Life Sciences ]
  • Sang-Young Lee [ Biotechnology Division. Gyeongsangnam Province Advanced Swine Research Institute ]
  • Choon-Keun park [ college of Bioengineering, Dalian University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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