Earticle

초록

영어

The purpose of the present study was to establish culture conditions for the in vitro study of the neonatal piglet Sertoli cell. Isolation for the culture of Sertoli cell was established using collagenase and pancreatin digestion of testicular tissues. The effects of various culture media, fetal bovine serum (FBS), follicular stimulating hormone (FSH), epidermal growth factor (EGF) and insulin-transferrin-sodium selenite (ITS) on growth of neonatal piglet Sertoli cells were investigated. The mitogenic effects of Dulbecco’s modified Eagle’s medium + Ham’s F-12 medium was higher than other media used in this experiment. The addition of 1% FBS in cultures was necessary for attachment of Sertoli cell clusters. However, except FBS and EGF, FSH and ITS did not stimulate Sertoli cell proliferation. When Sertoli cells isolated from neonatal piglets were cultured in Dulbecco’s modified Eagle’s medium + Ham’s F-12 medium supplemented with 1% FBS, FSH, EGF and ITS, the yield and plating efficiency of Sertoli cells were largely increased. Confluency of Sertoli cells was reached as early as 4 days of culture. The method described here reduces or eliminates many of the drawbacks of the conventional procedures used to isolate and culture of Sertoli cells, thus providing a useful tool in studies of growth kinetics and regulation of cell proliferation in vitro.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

저자

• Md, Anower Jabed [ Department of Molecular Biology, Dong-Eui Univercity ]
• Tania Kamel [ Department of Molecular Biology, Dong-Eui Univercity ]
• Byung-Ki Kim [ Department of Molecular Biology, Dong-Eui Univercity ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636