Earticle

초록

영어

Background: Radiation-induced pneumonitis and pulmonary fibrosis are common dose-limiting complications in patients receiving radiotherapy for lung, breast, and lymphoid cancers. In this study, we investigated the characteristics of effective immune cells related to pneumonitis and fibrosis after irradiation. Materials and Methods: After anesthesia, the whole thorax of C57BL/6 mice was irradiated at 14 Gy. The lung tissue and bronchoalveolar lavage fluid were collected at defined time points post-irradiation for the determination of histological and immunohistochemical analysis and inflammatory cell population infiltrated into the lung. Results and Discussion: Whole thoracic irradiation increased the deposition of extracellular matrix (ECM), lung weight, and pleural effusions, which started to die from 4 months later. At 4 months after irradiation, the numbers of macrophages and lymphocytes as well as neutrophils were increased dramatically in the lung. Interestingly, the macrophages that were recruited into the lung after irradiation had an enlarged foamy morphology. In addition, the expressions of chemokines (CCL-2, CCL-3, CXCL-10) for the attraction of macrophages and T cells were higher in the lung of irradiated mice. The high expressions of these chemokines were sustained up to 6 months following irradiation. In thoracic irradiated mice, infiltrated macrophages into the lung had the high levels of Mac-3 antigens on their surface and upregulated the hallmarks of alternatively activated macrophages such as arginase-1 and CD206. Furthermore, the levels of IL-4 and IL-13 were higher in a BAL fluid of irradiated mice. Conclusion: All results show that thoracic irradiation induces to infiltrate various inflammation- related immune cells, especially alternatively activated macrophages, through enhancing the expression of chemokines, suggesting that alternatively activated macrophages are most likely important for leading to pulmonary fibrosis.

목차

ABSTRACT
 Introduction
 Materials and Methods
  1. Animals and Ethics Statement
  2. Thoracic irradiation
  3. Survivability and lung weight after irradiation
  4. Determination of the volume of pleural exudates
  5. Masson’s trichrome blue staining
  6. cDNA microarray analysis
  7. Preparation of total leukocytes from bronchoalvelolar lavage (BAL) fluid and differential counting
  8. Preparation of total leukocytes from the lung tissue and flow cytometry analysis
  9. Immunohistochemistry analysis
  10. Analysis of the mRNA expression in the reverse transcriptase (RT)-polymerase chain reaction(PCR)
  11. Western blotting
  12. Measurement of cytokines in BAL fluid
 Results and Discussion
  1. Thoracic irradiation-induced pneumonitis and pulmonary fibrosis
  2. Thoracic irradiation induces the accumulation of extracellular matrix in the lung
  3. Thoracic irradiation increases the recruitment of various immune cells into BAL fluid and the lung
  4. Thoracic irradiation enhances the chemokines expression for the attraction of macrophages
  5. Recruited macrophages after thoracic irradiation have Mac-3 antigen on their surface
  6. Recruited macrophages after thoracic irradiation are alternatively activated macrophages, M2 macrophages
 Conclusion
 Acknowledgements
 References

키워드

저자

• Hae-Ran Park [ Radiation Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea ] Corresponding author
• Sung-Kee Jo [ Radiation Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea ]
• Uhee Jung [ Radiation Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  방사선방어학회지 [Journal of Radiation Protection and Research]

• 간기

  계간

• pISSN

  2508-1888

• 수록기간

  1976~2026

• 등재여부

  KCI 등재,SCOPUS

• 십진분류

  KDC 559 DDC 629