Earticle

초록

영어

Atopic dermatitis (AD) is a chronic relapsing allergic inflammatory skin disease that currently affects millions of children and adults worldwide. Drugs used to treat these inflammatory diseases include anti-histamines, corticosteroids and calcineurin inhibitors but these drugs have their limitations such as adverse effects with their long-term usage. Thus, researcher’s interest in several alternative and complementary therapies are continually growing and balneotherapy is one of these approaches. Therefore, we investigate the bathing effect of high concentration mineral spring water (HMW) on redox balance and immune modulation in 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis like inflammation in hairless mice. We induced AD-like inflammation by application of DNCB on the dorsal skin of female skh-1 hairless mice. The mice were treated with 100% pure HMW (PHMW) and 10% diluted HMW (DHMW) through bathing once a day for 4 weeks. Tacrolimus ointment (0.1%) was used as positive control (PC) and only DNCB treatment as negative control (NeC) group. The severity of skin lesion inflammation was assessed through clinical scoring and observing scratching behavior. Levels of immunoglobulin E (IgE) and inflammatory cytokines in serum were detected by ELISA and multiplex bead array system, and the levels of oxidative stress-related biomarkers and antioxidant enzyme were also measured. We found that HMW significantly decreased the scratching behavior in PHMW and DHMW groups at the 2nd week and in PHMW group at 4th week compared to NeC group. Likewise, serum IgE level was significantly decreased in DHMW group as compared to NeC group. In line, the level of inflammatory cytokines in serum such as interleukin (IL)-1 , IL-13 and tumor necrosis factor- β α were significantly inhibited in PHMW and DHMW groups compared to NeC group. In parallel, total reactive oxygen species (ROS) of serum level was significantly decreased in PHMW treatment groups compared to NeC group. Consistently, serum malondialdehyde (MDA) level in PHMW group was lower than in NeC group. By contrast, glutathione peroxidase (GPx) activity was significantly enhanced in PHMW than NeC. Collectively, our study indicates a balneotherapeutic effect of HMW on DNCB-induced AD like inflammation in hairless mice via immunomodulation and redox balance.

키워드

저자

• Johny Bajgai [ Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea, Department of Global Medical Science, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea ]
• Ailyn Fadriquela [ Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea, Department of Global Medical Science, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea ]
• Jesmin Ara [ Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea, Department of Global Medical Science, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea ]
• Rahima Begum [ Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea, Department of Global Medical Science, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea ]
• Md Faruk Ahmed [ Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea, Department of Global Medical Science, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea ]
• Cheol-Su Kim [ Department of Microbiology, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea ]
• Soo-Ki Kim [ Department of Microbiology, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea ]
• Kwang-Yong Shim [ Department of Internal Medicine, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea ] Corresponding author
• Kyu-Jae Lee [ Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea, Institute for Poverty Alleviation and International Development (IPAID), Yonsei University, Wonju Campus, Wonju, Gangwon 26493, Republic of Korea. ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국물학회지 [Korean Journal of Waters]

• 간기

  부정기

• pISSN

  2233-4653

• 수록기간

  2010~2021

• 십진분류

  KDC 539 DDC 628