Earticle

초록

영어

Since the first report of the oak wilt disease at 2004 in Korea, the disease distributed over Korean peninsula and are still giving severe damages. The management of oak wilt disease in Korea has mainly focused on the control of insect vector, Platypus koryoensis. Neverthless the effective method for evaluating the pathogenicity of the pathogen, Raffaelea quercus-mongolicae (Rqm), and for screening chemical or biological agents with strong inhibitory activity against the pathogen, is absolutely necessary, an reliable method is not available so far. This study was conducted to develop the effective method for evaluating the pathogenicity of Rqm in oak trees. The culture suspensions of Rqm were artificially injected to the saplings of Quercus acutissima by using ChemJet tree injector. Three months after treatments, the treated saplings were cut and dipped into 1% fuchsin acid solution. There were significant differences in non-conductive area (%), discoloration area (%) and vertical discoloration length between the pathogen-injected and distilled water-injected control treatments. These results indicated that the pathogen is the causal agent for the dysfunction of water conductive tissue, which will finally result in wilt symptom. Re-isolation of the pathogen and PCR detection using specific primers for the pathogen also confirmed the presence of Rqm in the sapwood chips of the pathogen-injected saplings. These observations would be greatly applied to other related researches for evaluating the pathogenicity of tree wilt pathogens and biocontrol efficacy of the selected antagonistic microorganisms, in case that the wilt symptom is not easily shown by artificial inoculation of the causal agent.

목차

Abstract
 Introduction
 Materials and Methods
  Tree saplings used
  Preparation of inoculum and inoculation
  External Symptom development
  Cutting of the treated saplings and staining with fuchsin acid solution
  Non-conductive area (NCA), discolored area (DA) and length (VDL)
  Re-isolation of the pathogen inoculated
  PCR detection of the pathogen from inoculated saplings
 Results and Discussion
  ChemJet trunk injection of the pathogen and sterilizedwater
  External symptom development
  Non-conductive area (NCA), discolored area (DA) and length (VLD)
  Re-isolation of the pathogen inoculated
  PCR detection of the pathogen from inoculated saplings
 Acknowledgements
 References

키워드

저자

• Su Hee Yi [ Tree Pathology and Mycology Lab., College of Forest and Environment Science, Kangwon National University, Chuncheon 24341, Republic of Korea ]
• Jin Heung Lee [ Forest Environment Research Institute, Osan 12408, Republic of Korea ]
• Sang Tae Seo [ National Institute of Forest Science, Seoul 02455, Republic of Korea ]
• Jong Kyu Lee [ Tree Pathology and Mycology Lab., College of Forest and Environment Science, Kangwon National University, Chuncheon 24341, Republic of Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634