Earticle

초록

영어

Dipsaci Radix was described as the roots of Dipsacus asperoides in the Korean pharmacopoeia, namely Sok-dan. In Korea, a herbaceous plant species Dipsacus japonicus belong to the same genus also distributed. Unfortunately, due to the morphological similarity of the areal parts and dried root, the accurate identification of these two plant is very difficult depending on the morphological features. Therefore, we introduced the genetic tools to distinguish D. asperoides and D. japonicus at the species levels based on the nuclear ribosomal RNA gene (nrDNA) internal transcribed spacer 2 (ITS2) region sequences. In comparison of ITS2 sequences using 7 and 2 samples of D. asperoides and D. japonicus, respectively, we identified seven distinct species-specific single nucleotide polymorphisms (SNPs), namely marker nucleotides, enough to discriminate both two plant species. Furthermore, these two species were clearly classified into distinct two group depending on species in the phylogenetic analysis of 7 Dipsacus species. These results indicate that the sequence variability and species-specific nucleotide substitutions of ITS2 region are useful genetic marker for identifying authentic Dispsaci Radix.

목차

Abstract
 서론
 본론
  1. 재료 및 방법
  2. 결과 및 고찰
 결론
 감사의 글
 참고문헌

키워드

저자

• 박인규 [ Inkyu Park | 한국한의학연구원 K-herb 연구단 ]
• 노푸름 [ Pureum Noh | 한국한의학연구원 K-herb 연구단 ]
• 김욱진 [ Wook Jin Kim | 한국한의학연구원 K-herb 연구단 ]
• 양선규 [ Sungyu Yang | 한국한의학연구원 K-herb 연구단 ]
• 문병철 [ Byeong Cheol Moon | 한국한의학연구원 K-herb 연구단 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한약정보연구회지 [Korean Herbal Medicine Informatics]

• 간기

  반년간

• pISSN

  2288-5161

• eISSN

  2288-5293

• 수록기간

  2013~2025

• 십진분류

  KDC 519 DDC 610