Earticle

초록

영어

Xenotransplantation is proposed as a solution to the problem of organ shortage. However, transplantation of xenogeneic organs induces an antigen-antibody reaction in α-1,3-gal structure that are not present in humans and primates, and thus complement is also activated and organs die within minutes or hours. In this study, we used FasL gene, which is involved in the immune response of NK cell, and US11, which suppresses MHC Class I cell membrane surface expression, to inhibit cell mediated rejection in the interspecific immunity rejection, and also hDAF(CD55) was introduced to confirm the response to C3 complement. These genes were tranfeced into Korean native pig fetal fibroblasts using pCAGGS vector. And cytotoxicity of NK cell and human complement was confirmed in each cell line. The US11 inhibited the cytotoxicity of NK cell and, in addition, the simultaneous expression of US11 and Fas ligand showed excellent suppress to T-lymphocyte cytotoxicity, hDAF showed weak resistance to cytotoxicity of natural killer cell but not in CD8+ CTLs. Cytotoxicity study with human complement showed that hDAF was effective for reducing complement reaction. In this studies have demonstrated that each gene is effective in reducing immune rejection.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS & METHODS
  Production of Expression Vector
  Establishment of Transgene Cell Lines
  Analysis of NK Cell Cytotoxicity
  Analysis of Cytotoxicity with CD8+ CTLs
  Analysis of Human Complement Serum
  Statistical Analysis
 RESULTS
  Vector Screening
  Identification and Efficiency of Transfected Cells with Multiple Genes
  Cytotoxicity Test of Transformed Cell Line Using NK Cell
  Cytotoxicity of Human Complement Serum
 DISCUSSION
 REFERENCE

키워드

저자

• Jung Won Kang [ Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea ]
• Hyeon Yeong Shin [ Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea ]
• Reza K. Oqani [ Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea ]
• Tao Lin [ Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea ]
• Jae Eun Lee [ Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea ]
• So Yeon Kim [ Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea ]
• Joo Bin Lee [ Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea ]
• Dong Il Jin [ Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636