Earticle

초록

영어

Until today, success in germline cells and tissue cryopreservation is limited mainly due to the poor understanding of the complex physiological processes can lead to cell damage during cryopreservation. Germline cells, from both male and female, have unique ability to differentiate into one or more cell lines and thus it becomes a crucial point to store them in subzero temperature with the minimal damage of their functional properties and maximum recovery of unchanged and viable cells when thawed. In the past three decades, a vast research has been performed using various different animal models which in fact have led to development of new methodologies and optimization of older one. However, successful use of animal model has provided the opportunity in research with human germline cells and tissues preservation, but not in all the cases. Therefore, the use of new cryo-protective chemicals and modified protocols have been often found in different groups of researchers based on the types, physical structures, utility and animal species of the specimens to be cryopreserved. This review discusses about the basics of different types of cryopreservation methodologies and commonly used optimized protocols and cryoprotectants for germline cells and tissues preservation.

목차

ABSTRACT
 INTRODUCTION
 CRYOPRESERVATION METHODS
  Slow Freezing
  Vitrification
  Freeze Drying
 METHODS APPLICABLE FOR THE CRYOPRESERVATION OF GAMETES, GERMLINE CELLS AND TISSUES
  Cryopreservation of Spermatozoa
  Cryopreservation of Oocyte
  Cryopreservation of Spermatogonia
  Cryopreservation of Testis Tissue
  Cryopreservation of Ovarian Tissue
 CONCLUSION
 REFERENCES

키워드

저자

• Polash Chandra Karmakar [ Department of Animal Science & Technology, Chung-Ang University, Anseong 456-756, Republic of Korea ]
• Sang-Eun Jung [ Department of Animal Science & Technology, Chung-Ang University, Anseong 456-756, Republic of Korea ]
• Buom-Yong Ryu [ Department of Animal Science & Technology, Chung-Ang University, Anseong 456-756, Republic of Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636