Earticle

초록

영어

In all mammalian species, progesterone is essential to both the preparation for, and maintenance of, pregnancy. The 20α-hydroxysteroid dehydrogenase (20α-HSD) enzyme predominantly converts progesterone into its biologically inactive form 20α-hydroxyprogesterone, thereby regulating its activity. Thus, to directly assess sexual maturation in the MediKinetics micropig®, we analyzed the concentration of the steroid hormones progesterone and estradiol during the estrous cycle. Our results show that the progesterone level exhibited by the analyzed micorpig® was low at the beginning of the estrous cycle, and then abruptly increased to 30.32±10.0 ng/mL and 46.37±11.0 ng/mL by days 9 and 11 of the cycle, respectively. It reached the highest level 55.87±3.5 ng/mL on day 13 of the estrous cycle, before decreasing to 46.58± 13.1 ng/mL and 10.0±7.6 ng/mL by days 15 and 17 of the cycle, respectively. In contrast, the estradiol level was shown to be highest (27.13±11.2 ng/mL) at the initiation of the estrous cycle, after which point it decreased to 13.29±6.5 ng/mL and 10.94±5.9 ng/mL by days 4 and 5 of the estrous cycle, respectively. By day 17 of the estrous cycle, the estradiol level decreased to 4.13±7.6 ng/mL. We anticipate that these results will provide useful information to enable the study of human ovulation and reproductive physiology using the MediKinetics micoripig® as a model system. We recommend further investigation to elucidate the functional mechanisms underlying the regulation of sexual maturation in the MediKinetics micropig®.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Materials
  Collection of Blood Samples during the Estrous Cycle
  Steroid Hormone Analysis
 RESULTS
  Progesterone Concentration during Estrous Cycle
  Estradiol Concentration during Estrous Cycle
 DISCUSSION
 ETHICS
 FUNDING
 REFERENCES

키워드

저자

• Hun-Ki Seong [ Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Ansung 17579, Republic of Korea ]
• Kyeong-Seok Seo [ Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Ansung 17579, Republic of Korea ]
• Jeong-Su Kim [ Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Ansung 17579, Republic of Korea ]
• Chang-Gi Her [ MediKinetics, PyeongTaek 17792, Republic of Korea ]
• Myung-Hwa Kang [ Department of Food Science and Nutrition, Hoseo University, Asan 31499, Republic of Korea ]
• Bo-Woong Sim [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang 28116, Republic of Korea ]
• Jong-Taek Yoon [ Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Ansung 17579, Republic of Korea ]
• Kwan-Sik Min [ Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Ansung 17579, Republic of Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636