Earticle

초록

영어

Cerebral palsy (CP) is a chronic disorder characterized by physical dis-ability and disruption of brain function. We evaluated the effects of treadmill exercise on motor and memory functions in relation with phosphatidylinositol 3-kinase (PI3K)-Akt pathway using CP rat model. Rota-rod test, step-down avoidance task, 5-bromo-2’-deoxyuridine (BrdU) immunohistochemistry, and western blot for synapsin I, postsyn-aptic density-95 (PSD-95), PI3K, Akt, and glycogen synthase kinase-3β (GSK-3β) were performed. CP was induced by maternal lipopolysac-charide (LPS)-injection with sensorimotor restriction. Five weeks after birth, the rats in the exercise groups were made to run on the treadmill for 30 min per one day, 5 times a week, during 4 weeks. Motor and memory functions were impaired in the LPS-induced CP rats and tread-mill exercise increased motor and memory functions in the CP rats. Cell proliferation in the hippocampus was suppressed in the LPS-induced CP rats and treadmill exercise increased hippocampal cell proliferation in the CP rats. Expressions of synapsin I, PSD-95, phosphorylated (p)-PI3K, and p-Akt were decreased in the LPS-induced CP rats and tread-mill exercise enhanced the expressions of synapsin I, PSD-95, p-PI3K, and p-Akt in the CP rats. GSK-3β expression was increased in the LPS-induced CP rats and treadmill exercise suppressed GSK-3β ex-pression in the CP rats. The present results suggest that treadmill exer-cise might improve motor and memory functions through activation of PI3K-Akt pathway.

목차

Abstract
 INTRODUCTION
 MARERIALS AND METHODS
  Animals and treatments
  Treadmill exercise protocol
  Rota-rod test
  Step-down avoidance task
  Tissue preparation
  Immunohistochemistry for 5-bromo-2’-deoxyuridine
  Western blotting
  Data analysis
 RESULTS
  Motor function
  Memory function
  Cell proliferations in the hippocampal dentate gyrus
  Synapsin I, PSD-95, p-PI3K, p-Akt, GSK-3β expressions in the hippocampus
 DISCUSSION
 CONFLICT OF INTEREST
 ACKNOWLEDGMENTS
 REFERENCES

키워드

저자

• Sun-Young Jung [ Department of Physical Therapy, Hosan University, Gyeongsan, Korea ]
• Dae-Young Kim [ Department of Sports Healthcare, College of Humanities & Social Sciences, Inje University, Gimhae, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613