Earticle

초록

영어

Glycosylation is one of the most important post-translational modifications of protein, which plays a key role on the stability and immunogenicity of therapeutic monoclonal antibodies (mAbs). Alteration in N-glycoforms may significantly modify the biological activity of mAbs. The unexpected and heterogeneous N-glycoforms are often reported, because it is affected by the culture medium, the efficiency of protein expression, and the physiological status of the host cells. Thus, accurate identification and quantification of their N-glycoform are necessary in order to control the production processes. In this study, IgG (Human serum), two NIST (National institute of standards and technology), and three unknown therapeutic mAb samples were analyzed by LC-MS/MS and GPA (GlycoProteome Analyzer) system1. As a result, the complex types of N-glycoforms were identified in IgG and unknown sample A, whereas different high-mannose and hybrid types of N-glycoforms were identified in two NIST samples and unknown sample B and C. The quantitative patterns of N-glycoform were similar between IgG and unknown sample A. The hybrid type and high-mannose type of N-glycoform were quantitatively increased in two NIST samples and unknown sample B and C, respectively. This analytical method will be useful for verification of safety and efficacy in various biopharmaceutical products such as therapeutic monoclonal antibodies.

저자

• Hyun Kyoung Lee [ Biomedical Omics Group, Korea Basic Science Institute, Cheongju, Republic of Korea, Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Republic of Korea ]
• Ju Yeon Lee [ Biomedical Omics Group, Korea Basic Science Institute, Cheongju, Republic of Korea ]
• Gun Wook Park [ Biomedical Omics Group, Korea Basic Science Institute, Cheongju, Republic of Korea ]
• Young Hee Ahn [ Department of Biomedical Science, Cheongju University, Cheongju, Republic of Korea ]
• Jin Young Kim [ Biomedical Omics Group, Korea Basic Science Institute, Cheongju, Republic of Korea ]
• Jong Shin Yoo [ Biomedical Omics Group, Korea Basic Science Institute, Cheongju, Republic of Korea, Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Republic of Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614