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In-depth Analysis of Site-specific N-glycosylation in Vitronectin from Human Plasma by Tandem Mass Spectrometry with Immunoprecipitation

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    2017 한국당과학회 동계학술대회 (2017.01)바로가기
  • 페이지
    pp.66-66
  • 저자
    Heeyoun Hwang, Ju Yeon Lee, Hyun Kyoung Lee, Gun Wook Park, Hoi Keun Jeong, Myeong Hee Moon, Jin Young Kim, Jong Shin Yoo
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A294824

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원문정보

초록

영어
The characterization of site-specific micro-heterogeneity in glycoprotein is very important for understanding cell biology and disease processes. Vitronectin is well known to be a multi-functional glycoprotein in blood and the extracellular matrix, which is related to hepatocellular carcinoma (HCC). Here, we systematically analyzed the site-specific N-glycopeptides of vitronectin in human plasma by tandem mass spectrometry combined with immunoprecipitation and HILIC enrichment. Vitronectin was purified with immunoprecipitation by monoclonal antibody from plasma and digested to tryptic N-glycopeptides. Then, enrichment with HILIC materials was used, and followed by analysis with nano-LC/MS/MS. The sequences of N-glycopeptides were identified from the mass spectra by high-energy C-trap dissociation (HCD) and collision-induced dissociation (CID). In HCD mode, oxonium ions were used for recognizing glycopeptides and y ions for sequencing the peptide backbone. In CID mode, Y ions were used for characterizing their glycoforms. As a result, total 17 site-specific N-glycopeptides were completely identified at all of three N-glycosylation sites of vitronectin in human plasma, including 12 N-glycopeptides first reported. Finally, we specifically found that three hybrid and four complex glycopeptides of tri-antennary forms with outer-fucosylation increased in HCC human plasma.

저자

  • Heeyoun Hwang [ Biomedical OMICS Group, Korea Basic Science Institute, Chungbuk, Republic of Korea ]
  • Ju Yeon Lee [ Biomedical OMICS Group, Korea Basic Science Institute, Chungbuk, Republic of Korea ]
  • Hyun Kyoung Lee [ Biomedical OMICS Group, Korea Basic Science Institute, Chungbuk, Republic of Korea, Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Republic of Korea ]
  • Gun Wook Park [ Biomedical OMICS Group, Korea Basic Science Institute, Chungbuk, Republic of Korea ]
  • Hoi Keun Jeong [ Biomedical OMICS Group, Korea Basic Science Institute, Chungbuk, Republic of Korea, Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Republic of Korea ]
  • Myeong Hee Moon [ Department of Chemistry, Yonsei University, Seoul, Republic of Korea ]
  • Jin Young Kim [ Biomedical OMICS Group, Korea Basic Science Institute, Chungbuk, Republic of Korea ]
  • Jong Shin Yoo [ Biomedical OMICS Group, Korea Basic Science Institute, Chungbuk, Republic of Korea, Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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