Seong Cheol Kim, Ji Won Choi, Hyeon Jung Kim, Jun Il Kim, Yong Il Park
언어
영어(ENG)
URL
https://www.earticle.net/Article/A294812
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원문정보
초록
영어
Capsosiphon fulvescens (commonly called Maesaengyi in Korea), a green algae, has been traditionally used in Korea for centuries as a functuinal food. Polysaccahrides (SPS-CF, 385 kDa) isolated from Capsosiphon fulvescens have been shown to exhibit various biological activities, including anti-coagulanting, immune modulating and anti-cancer properties. However, anti-osteoporosis activity of SPS-CF has been rarely explored. In this study, we investigated its anti-osteoporotic effects using RAW 264.7 osteoclastic cells. The results showed that SPS-CF have been dose-dependently reduced the TRAP activity in the RAW 264.7 cells, with an approximately 59 % reduction at 50 μg/ml, while SPS-CF did not reduced cell viability. The SPS-CF treatment reduced actin organization in RAW 264.7 cells and significantly decreased protein level of Carbonic anhydraseⅡ. The inhibition of osteoclast differentiation shown to be mediated by suppression of RANKL signaling pathway, as evidenced by decreased TRAF6, gelsolin and phosphorylation of Src. These results SPS-CF demonstrated for the first time that SPS-CF inhibits the osteoclast differentiation, suggestubg that SPS-CF can be a functional ingredient for health beneficial foods or therapeutic agents to treat osteoporosis.
저자
Seong Cheol Kim [ Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do 14662, Republic of Korea ]
Ji Won Choi [ Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do 14662, Republic of Korea ]
Hyeon Jung Kim [ Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do 14662, Republic of Korea ]
Jun Il Kim [ Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do 14662, Republic of Korea ]
Yong Il Park [ Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do 14662, Republic of Korea ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.