You Na Seo, Dong Hoon Kwak, Soung-Yun Heo, Won Seok Ju, Hyun Gyu Lim, Seo Yi Lee, Sun-Uk Kim, Ji-Su Kim, Kyu-Tae Chang, Young-Kug Choo
언어
영어(ENG)
URL
https://www.earticle.net/Article/A294745
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원문정보
초록
영어
Gangliosides are glycolipid having sialic acid. It is present in the central nervous system and involved in multiple functions of cell membranes and nerve function. However, roles of gangliosides in the differentiation of mesenchymal stem cells (MSCs) is unclear. Therefore, we investigated the effects of ganglioside in neuronal differentiation of adipose-derived MSCs (AD-MSCs). In the differentiated neuronal cells from AD-MSCs. we observed the expression of neuronal markers (MAP-2 and GFAP) and the high levels of mRNA expression of neuron-specific genes. Also, The expression of ganglioside GM2 and GD3 were significant increased in neuronal differentiation of AD-MSCs. In addition, this study showed that differentiation of AD-MSCs into neuronal cells significant decrease in the knockdown of UDP-glucose ceramide glucosyltransferase (UGCG) which well known as first step of ganglioside synthase and beta-1,4-N-acetyl-galactosaminyl transferase 1(B4GALNT1) gangllioside GM2 synthase. The results indicate that ganglioside GM2 and GD3 might be important roles in the neural differentiation of mini-pig AD-MSCs.
저자
You Na Seo [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk 570-749, Korea ]
Dong Hoon Kwak [ Institute for glycoscience, Wonkwang University, Iksan, Jeonbuk 570-749, Korea ]
Soung-Yun Heo [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk 570-749, Korea ]
Won Seok Ju [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk 570-749, Korea ]
Hyun Gyu Lim [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk 570-749, Korea ]
Seo Yi Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk 570-749, Korea ]
Sun-Uk Kim [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB), O-chang, Chungbuk 363-883, Korea ]
Ji-Su Kim [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB), O-chang, Chungbuk 363-883, Korea ]
Kyu-Tae Chang [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB), O-chang, Chungbuk 363-883, Korea ]
Young-Kug Choo [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk 570-749, Korea, Institute for glycoscience, Wonkwang University, Iksan, Jeonbuk 570-749, Korea ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.