Kyung-Hee Jung, Min-Jeong Kim, Hye Sun Hwang, Myo-Jeong Kim, Jae Cherl Kim, Heeseob Lee
언어
영어(ENG)
URL
https://www.earticle.net/Article/A294743
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
Various native and hydroxyethylated starch granules were reacted as substrates for a debranching enzyme, pullulanase, from Bacillus acidopullulyticus , and the kinetic mechanism was investigated in initial rate studies. The apparent K m values of the native starch hydrolysis reactions decreased in the order of rice, corn, and potato, whereas V max remained constant for all starches. Assuming that starch granules were spherical, the double reciprocal of the initial rates of the hydrolysis reactions along with the surface area of the three native starch granules could be described in a linear function. Therefore, enzymatic hydrolysis of native starches during the initial stage is mainly affected by particle size of starch granules. In case of hydroxyethylated corn starch (HES) and native corn starch, the apparent K m values of the native corn starch and HES hydrolysis reactions showed similar values, but V max of HES was higher than that of native corn starch, which was resulted from reduced activation energy of HES toward a debranching enzyme. From these results, the surface area as well as particle size of granules have important effects on the initial rate at which native starch is digested by debranching enzyme. Also, we could modulate the enzyme activity toward native starch by modifying the surface of native starches.
저자
Kyung-Hee Jung [ Dept. of Food Science and Nutrition, Pusan National University, Busan 46241, Korea ]
Min-Jeong Kim [ Dept. of Food Science and Nutrition, Pusan National University, Busan 46241, Korea ]
Hye Sun Hwang [ Dept. of Food Science and Nutrition, Pusan National University, Busan 46241, Korea ]
Myo-Jeong Kim [ Dept. of Food and Life Science, Inje University, Gyeongnam 50834, Korea ]
Jae Cherl Kim [ Dept. of Food and Life Science, Inje University, Gyeongnam 50834, Korea ]
Heeseob Lee [ 이희섭 | Dept. of Food Science and Nutrition, Pusan National University, Busan 46241, Korea ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.