Earticle

초록

영어

In plants, α1,3-fucosyltransferase (FucT) catalyzes the transfer of fucose from GDP-fucose to asparagine-linked GlcNAc of the N-glycan core in the medial Golgi. To explore the physiological significance of this processing, we isolated two Oryza sativa (rice) mutants (fuct-1 and fuct-2) with loss of FucT function. Biochemical analyses of the N-glycan structure confirmed that α1,3-fucose is missing from the N-glycans of allelic fuct-1 and fuct-2. Compared with the wild-type cv Kitaake, fuct-1 displayed a larger tiller angle, shorter internode and panicle lengths, and decreased grain filling as well as an increase in chalky grains with abnormal shape. The mutant allele fuct-2 gave rise to similar developmental abnormalities, although they were milder than those of fuct-1. Restoration of a normal tiller angle in fuct-1 by complementation demonstrated that the phenotype is caused by the loss of FucT function. Both fuct-1 and fuct-2 plants exhibited reduced gravitropic responses. Expression of the genes involved in tiller and leaf angle control was also affected in the mutants. We demonstrate that reduced basipetal auxin transport and low auxin accumulation at the base of the shoot in fuct-1 account for both the reduced gravitropic response and the increased tiller angle.

저자

• Rikno Harmoko [ Division of Applied Life Science (BK21+ program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Korea ]
• Jae Yong Yoo [ Division of Applied Life Science (BK21+ program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Korea ]
• Ki Seong Ko [ Division of Applied Life Science (BK21+ program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Korea ]
• Nirmal Kumar Ramasamy [ Division of Applied Life Science (BK21+ program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Korea ]
• Bo Young Hwang [ Division of Applied Life Science (BK21+ program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Korea ]
• Eun Ji Lee [ Division of Applied Life Science (BK21+ program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Korea ]
• Ho Soo Kim [ Division of Applied Life Science (BK21+ program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Korea ]
• Kyung Jin Lee [ Integrative Omics Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon ]
• Doo-Byoung Oh [ Integrative Omics Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon ]
• Dool-Yi Kim [ Crop Function Division, National Institute of Crop Science, Rural Development Administration, 181 Hyeoksin-ro, Wanju-gun, Jeollabuk-do 55365, Korea ]
• Sanghun Lee [ Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA ]
• Yang Li [ Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA ]
• Sang Yeol Lee [ Division of Applied Life Science (BK21+ program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Korea ]
• Kyun Oh Lee [ Division of Applied Life Science (BK21+ program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614