Sarang Cho, Juhee Shin, Seong Cheol Kim, Yong Il Park
언어
영어(ENG)
URL
https://www.earticle.net/Article/A294733
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원문정보
초록
영어
In this study, we extracted water soluble polysaccharides from the defatted Korean edible nut cake by boiling for 3 h in water and purified by ethanol precipitation and DEAE-cellulose column chromatography. The monosaccharide composition analysis showed that the purified polysaccharide (NP) is a galacturonoxyloarabinan type polysaccharide consisting of xylose (7.2, molar ratio), arabinose (8.8), and galacturonic acid (4.4) as major sugars and other minor sugars like glucose, galactose, rhamnose, and glucuronic acid. It's molecular mass was shown to be 1,274 kDa by size-exclusion HPLC. Then, we investigated the immunostimulating activity of NP in murine macrophage RAW 264.7 cells. The purified water soluble polysaccharide (NP) did not show any detectable level of cytotoxicity to RAW 264.7 cells in the concentration range tested (10-200 μg/ml) and rather, it promoted the cell proliferation. The NP stimulated the RAW 264.7 cells to produce the nitric oxide (NO) and increase the mRNA expression level of inducible nitric oxide synthase (iNOS). In addition, it induced the production of pro-inflammatory cytokines (TNF-α and Interleukin-6) and chemokines (RANTES and MIP-1α), which are largely known to play an important role in immune system. Taken together, these results suggest that NP can be considered a potential immunopotentiator and thus can be a potent ingredient of health beneficial functional foods or therapeutic agents.
저자
Sarang Cho [ Department of Biotechnology, The Catholic University of Korea ]
Juhee Shin [ Department of Biotechnology, The Catholic University of Korea ]
Seong Cheol Kim [ Department of Biotechnology, The Catholic University of Korea ]
Yong Il Park [ Department of Biotechnology, The Catholic University of Korea ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.