Earticle

초록

영어

Ginseng (Panax ginseng C.A. Meyer) is a highly valued herbal medicine that has been traditionally used to prevent or treat diverse medical disorders. Polysaccharides isolated from ginseng have been shown to exhibit various biological activities, including immune modulating, anti-diabetic, anti-cancer, and anti-obesity properties. However, the ginseng-derived oligosaccharides was rarely explored. In this study, we investigated the anti-cancer activity in A549 human lung cancer cells of ginseng polysaccharides (MW. 11.605 kDa) and its lower-molecular weight oligosaccharide (GOS, MW. 2.2 kDa) prepared by enzymatic degradation. The results showed that GOS dose-dependently reduced the cell viability of the A549 cells, with an approximately 55% reduction at 500 μg/ml, while GPS did not affect the cell viability. The GOS-induced A549 cell death was mediated by induction of cell cycle arrest at the sub.G1 (apoptotic) phase. GOS treatment also induced DNA fragmentation, depolarization of MMP, and reduction in Bcl-2 and pro-caspase-3 expression levels. Moreover, the anti-cancer effects of GOS were related to the MAPK signaling pathway, as evidenced by significantly decreased phosphorylation of ERK. These results clearly demonstrated for the first time that GOS inhibits the A549 human lung cancer cell growth via induction of cell cycle arrest and stimulation of mitochondria-dependent apoptotic cell death through inhibition of MAPK pathway. These findings suggested that GOS may have a strong therapeutic potential for the treatment of human lung cancer.

저자

• Jisun Lee [ Department of Biotechnology, The Catholic University of Korea ]
• Chang Won Lee [ Department of Biotechnology, The Catholic University of Korea ]
• Ji Won Choi [ Department of Biotechnology, The Catholic University of Korea ]
• Yong Il Park [ Department of Biotechnology, The Catholic University of Korea ]

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발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614