Woo Soo Jeong, Jin Young Bae, Dong Ho Seo, Jong Hyun Jung, Dong Hyun Jung, Cheon-Seok Park
언어
영어(ENG)
URL
https://www.earticle.net/Article/A294730
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원문정보
초록
영어
Amylosucrase (ASase, EC. 2. 4. 1. 4) forms an insoluble α-1,4-glucan polymer by transferring glucose molecules liberated from sucrose to its appropriate substrates, such as maltooligosaccharides, arbutin, and salicin. It was found that ASase could be attached into the insoluble α-1,4-glucan polymer after its catalytic work was done. In this study, therefore, ASase, a glucan-polymerization enzyme, was employed as a fusion partner for foreign proteins to make the foreign proteins be easily purified. Green fluorescent protein (GFP) and β-glucosidase were used as model fusion partner proteins. ASase-foreign gene cassettes were constructed and transformed into E. coli (BL21 DE3). As a result, it was found that ASase-fusion proteins were easily purified by a simple centrifugation since the majority of ASase-fusion proteins were present in the insoluble glucan polymer. In this study, optimal condition of polymer production was characterized.
저자
Woo Soo Jeong [ Graduate School of Biotechnology and Institute of Life Sciences & Resources Kyung Hee University, Yongin 17104, Republic of Korea ]
Jin Young Bae [ Graduate School of Biotechnology and Institute of Life Sciences & Resources Kyung Hee University, Yongin 17104, Republic of Korea ]
Dong Ho Seo [ Graduate School of Biotechnology and Institute of Life Sciences & Resources Kyung Hee University, Yongin 17104, Republic of Korea ]
Jong Hyun Jung [ Graduate School of Biotechnology and Institute of Life Sciences & Resources Kyung Hee University, Yongin 17104, Republic of Korea ]
Dong Hyun Jung [ Graduate School of Biotechnology and Institute of Life Sciences & Resources Kyung Hee University, Yongin 17104, Republic of Korea ]
Cheon-Seok Park [ Graduate School of Biotechnology and Institute of Life Sciences & Resources Kyung Hee University, Yongin 17104, Republic of Korea ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.