Earticle

초록

영어

Gangliosides are anionic glycosphingolipids that contain one to several sialic acid residues. They participate in diverse biological processes such as cell-cell recognition, and the modulation of membrane protein function. Nonetheless of the biological importance of gangliosides, there are few studies on gangliosides due to their structural complexity and the lack of sensitive analytical tools. In this study, we have performed detailed profiling of gangliosides present on various cancer cell membrane to differentiate the cancer origins and subtype using negative ion detection mode nano LC/MS combined with membrane enrichment technique. Cell surface gangliosides were extracted from eight cancer cell lines originated from breast, lung, cervical, and lymphatic tumor and then analyzed by nano LC/MS. Extracted gangliosides were assigned based on accurate mass and the structures were further elucidated using tandem MS. GD1, GM1 and GM3 were commonly observed as major gangliosides in all cancer cell lines having various ceramide portion. Hierarchical clustering based on Pearson correlation coefficients were also used to compare and classify each cell line. Cancer origins and molecular subtypes could be distinguished based on distinctive ganglioside profiles. These results demonstrate that significant differences in cancer cell surface gangliosides can be used for potential application toward cancer diagnosis and treatment.

저자

• Sumin Kim [ Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea, Asia-Pacific Glycomics Reference Site, Daejeon, Korea ]
• Jae Yun Hwang [ Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea, Asia-Pacific Glycomics Reference Site, Daejeon, Korea ]
• In Jung Ji [ Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea, Asia-Pacific Glycomics Reference Site, Daejeon, Korea ]
• Young Sam Kim [ Korea Research Institute of Bioscience and Biotechnology(KRIBB), Daejeon, Korea ]
• Hyun Joo An [ Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea, Asia-Pacific Glycomics Reference Site, Daejeon, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614