Earticle

초록

영어

The predominant N-glycan in plants is paucimannosidic N-glycan with core β1,2-xylose and α 1,3-fucose residues (PNGXF). Here, We hypothesized that additions of the core β1,2-xylose, α 1,3-fucose, and 6-arm β1,2-GlcNAc residues to the common acceptor (GlcNAcMan3GlcNAc2) are relatively determined by the different activity and/or substrate occupancy of the enzymes of the corresponding genes in plants. As a result, we describe a mechanism in Arabidopsis thaliana that effectively form the largest N-glycan in plants. Genetic and biochemical evidence suggest that the addition of the 6-arm β1,2-GlcNAc residue by N-acetylglucosaminyltransferase II (GnTII) is less effective than additions of the core β1,2-xylose and α1,3-fucose residues by XylT, FucTA, and FucTB in Arabidopsis thaliana. Moreover, analysis of gnt2 mutant and 35S: GnTII transgenic plants indicates that the additions of the core β1,2-xylose and α1,3-fucose residues to the common acceptor (GlcNAcMan3GlcNAc2) are partially inhibited by the addition of the 6-arm β1,2-GlcNAc residue. Our findings show that plants control the rate of the addition of the 6-arm GlcNAc residue to the common N-glycan acceptor as a mechanism to facilitate formation of the general N-glycans with Man3XylFucGlcNAc2 and GlcNAc2Man3XylFucGlcNAc2 structures by hexosaminidase activity and the regulated sharing of the common acceptor GlcNAcMan3GlcNAc2.

저자

• Eun Ji Lee [ Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University ]
• Jae Yong Yoo [ Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University ]
• Rikno Harmoko [ Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University ]
• Ki Seong Ko [ Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University ]
• Nirmal Kumar Ramasamy [ Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University ]
• Bo Young Hwang [ Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University ]
• Sang Yeol Lee [ Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University ]
• Kyun Oh Lee [ Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614