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Comprehensive Glycomic Analysis of Haptoglobin in Gastrointestinal Cancer

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    2016 한국당과학회 동계학술대회 (2016.01)바로가기
  • 페이지
    pp.17-17
  • 저자
    Sung Hyeon Lee, Serenus Hua, Myung Jin Oh, Seung-Yeol Park, Jin Man Kim, Hyun Joo An, Jung Hoe Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A294675

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원문정보

초록

영어
Aberrant glycosylation in serum proteins are associated with many human diseases, including various types of cancer. In this study, we developed a comprehensive glycomic approach involving immunoaffinity purification, lectin blotting, and advanced MS techniques to discover potential biomarkers for gastrointestinal cancer, especially focus on colon and gastric cancer. Haptoglobin (Hp), a target glycoprotein that displays aberrant glycosylation during cancer development, was initially screened by blotting with various lectins. To discover novel aberrant glycans in colon and gastric cancer, we have analyzed glycosylation status of haptoglobin by lectin blotting. In particular, aleuria aurantia lectin (AAL, which is specific to fucosyl residues) reactivity with cancer Hp was higher than control groups. Moreover, by LC/MS analysis following PNGaseF treatment for purified Hp, several aberrant glycans were detected in Hp from sera of colon and gastric cancer patients. Increased levels of antennary branching and reduced levels of high-mannose type structures were observed in cancer patients. In addition, enhanced fucosylation at Asn 241 of the four potential N-glycosylation sites of Hp in sera of cancer patients was especially identified by site-specific analysis. Our findings indicate that glycome profiling of Hp will be a useful platform for gastrointestinal cancer biomarker discovery, rapidly identifying a number of potential diagnostic markers for colon and gastric cancer.

저자

  • Sung Hyeon Lee [ Department of Biological Sciences, Korea Advanced Institute of Science and Technology ]
  • Serenus Hua [ Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea ]
  • Myung Jin Oh [ Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea ]
  • Seung-Yeol Park [ .Division of Rheumatology, Immunology, and Allergy, Brigham and Women’s Hospital, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA ]
  • Jin Man Kim [ Department of Pathology and Cancer Research Institute, Chungnam National University College of Medicine, Daejeon, Korea ]
  • Hyun Joo An [ Graduate School of Analytical Science and Technology, Chungnam National University, Division of Rheumatology, Immunology, and Allergy, Brigham and Women’s Hospital, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA ]
  • Jung Hoe Kim [ Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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