Earticle

초록

영어

Understanding of the molecular relationships in carbohydrate-protein interactions provides useful information on biological processes in living organisms and is also helpful for development of potent diagonostic systems. Synthesizing the specific complex glycans is important for analysis of interactions between glycans and their binding proteins. Particularly, on-chip enzymatic synthesis of complex glycans can be an efficient and useful tool because this can directly provide diverse glycans to use for analyzing carbohydrate-protein interactions on the chip. We prepared novel on-chip glycan synthesis platform in which glycan-conjugated single-stranded DNA was synthesized using DNA synthesizer and the synthesized carbohydrate-oligonucleotide conjugates (COCs) were immobilized onto the complementary single-stranded DNA-modified glass slide. Due to the property of DNA to be possible for reversible hybridization and denaturation under certain conditions, we effectively recovered the synthesized glycans from the chip, and optimized the synthesis conditions for target complex glycans. We anticipate that this novel on-chip complex glycan synthesis can be successfully used in a number of glycan-related studies including analysis of diverse carbohydrate-protein interactions and high throughput diagnosis.

저자

• Hye Ryoung Heo [ Department of Chemical Engineering, Pohang University of Science and Technology ]
• Chang Sup Kim [ School of Biotechnology and Graduate School of Biochemistry, Yeungnam University ]
• Jeong Hyun Seo [ School of Chemical Engineering, Yeungnam University ]
• Hyung Joon Cha [ 차형준 | Department of Chemical Engineering, Pohang University of Science and Technology ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614