버섯 세균성회색무늬병균(Pseudomonas agarici)에 대한 길항 세균 Alcaligenes sp. HC12의 대량배양을 위한 최적 배양조건
Optimum cultivation conditions for mass production of antagonistic bacterium Alcaligenes sp. HC12 effective in antagonistic of browning disease caused by Pseudomonas agarici
This study was conducted to investigate optimum conditions for mass production of ntagonistic microbes Alcaligenes sp. HC12. Alcaligenes sp. HC12 had a potent biological control agent to control browning disease caused by Pseudomonas agarici. Alcaligenes sp. HC12 markedly showed the antagonistic activity against Pseudomonas agarici, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the Alcaligenes sp. HC12, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 9.0 and 30o, respectively. The optimal concentration of medium elements for the growth of pathogen inhibitor bacterium(Alcaligenes sp. HC12) was determined as follows: 0.5% dextrine, 1.5% yest extract, 1.0% NaNO3, 0.5% KH2PO4, and 1.5% asparagine.
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ABSTRACT 서론 재료 및 방법 공시균주 및 배양 배양적온 초기 pH의 영향 영양원에 따른 길항미생물의 증식에 미치는 영향 결과 및 고찰 온도와 pH에 따른 영향 탄소원에 따른 영향 무기질소원에 따른 영향 유기질소원에 따른 영향 아미노산에 따른 영향 무기염류에 따른 영향 길항미생물 HC12의 최적 배양조건 적요 References