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Forensic DNA Analysis for Discrimination of Hybrid Fishes : A Case Report from National Forensic Service, South Korea

첫 페이지 보기
  • 발행기관
    한국과학수사학회 바로가기
  • 간행물
    과학수사학회지 KCI 등재 바로가기
  • 통권
    Vol.10 No.4 (2016.12)바로가기
  • 페이지
    pp.329-334
  • 저자
    Yoonjung Cho, Min-Hee Kim, Hyo Sook Kim, Chong Min Choung, Min Young Lee, Yang Han Lee, Dong Sub Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A290348

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원문정보

초록

영어
Ssangji carp is a hybrid fish produced by crossbreed of Carassius carassius and Cyprinus carpio (or Cyprinus carpio nudus). In Korea, import of the hybrid fishes is strictly prohibited because the hybrid fishes can destruct the ecosystem by disturbing food-chain as well as by transmitting exotic diseases. However, Ssangji carp produced in a foreign country is illegally being released to fishing places. Therefore we selected two target genes, recombination activating 1 (RAG1) and mitochondrial cytochrome b, for genetic identification of the hybrid fishes and analyzed base sequences of the genes. We found 6 different bases exist when we compared RAG1 gene sequences of C. carassius and C. carpio (or C. carpio nudus). In particular, the hybrid fishes showed double peaks on the 6 base positions indicating expression of both C. carassius and C. carpio (or C. carpio nudus). Maternal line of the fishes was identified by mitochondrial cytochrome b gene. This forensic study provides the forensic analysis method for discrimination of the hybrid fishes.

목차

Abstract
 Introduction
 Materials and Methods
  1. Collection of samples
  2. Extraction of genomic DNA and PCR
  3. Sanger type sequencing
 Results and Discussion
 Acknowledgements
 References

키워드

Hybrid fish Recombination activating 1 Cytochrome b Forensic analysis

저자

  • Yoonjung Cho [ Forensic DNA Division, National Forensic Service, Wonju, Gangwon-do, 26460 Korea ]
  • Min-Hee Kim [ Forensic DNA Division, National Forensic Service, Wonju, Gangwon-do, 26460 Korea ]
  • Hyo Sook Kim [ Forensic DNA Division, National Forensic Service, Wonju, Gangwon-do, 26460 Korea ]
  • Chong Min Choung [ Forensic DNA Division, National Forensic Service, Wonju, Gangwon-do, 26460 Korea ]
  • Min Young Lee [ Forensic DNA Division, National Forensic Service, Wonju, Gangwon-do, 26460 Korea ]
  • Yang Han Lee [ Forensic DNA Division, National Forensic Service, Wonju, Gangwon-do, 26460 Korea ]
  • Dong Sub Lee [ Forensic DNA Division, National Forensic Service, Wonju, Gangwon-do, 26460 Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국과학수사학회 [The Korean Academy of Scientific Criminal Investigation]
  • 설립연도
    2007
  • 분야
    자연과학>자연과학일반
  • 소개
    본회는 과학수사학 분야의 전문 인력 육성, 연구개발, 전문화 교육 및 인증, 회원 상호간의 친목을 목적으로 한다.

간행물

  • 간행물명
    과학수사학회지 [Journal of Scientific Criminal Investigation]
  • 간기
    계간
  • pISSN
    2466-1422
  • eISSN
    2466-1430
  • 수록기간
    2016~2017
  • 십진분류
    KDC 367 DDC 340

이 권호 내 다른 논문 / 과학수사학회지 Vol.10 No.4

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