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Effects of α-Linolenic Acid and Bovine Serum Albumin on Frozen-thawed Boar Sperm Quality during Cryopreservation

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 40 No 4 (2016.11)바로가기
  • 페이지
    pp.33-37
  • 저자
    Won-Hee Lee, Yong Hwangbo, Sang-Hee Lee, Hee-Tae Cheong, Boo-Keun Yang, Choon-Keun Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A289046

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초록

영어
This study was conducted to evaluate effect of α-linolenic acid (ALA) and bovine serum albumin (BSA) on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. The boar semen was collected by gloved- hand method and cryopreserved using freezing extender containing 3 ng/mL ALA and/or 20 μg/mL BSA. Cryopreserved boar sperms were thawed in 37℃ water-bath for 45 sec to analysis. Viability, acrosome reaction, and mitochondrial intact were analyzed using flow cytometry. In results, viability of frozen-thawed boar sperm was significantly higher in only ALA+BSA supplement group than control group (p<0.05), whereas there was no difference either in ALA or BSA supplement. However, acrosome reacted sperm in both of live and all sperm population were significantly decreased in all treatment groups than control (p<0.05). Interestingly, mitochondrial intact of boar sperm was enhanced in ALA and ALA+BSA groups compared with control (p<0.05). In this study, we showed that supplementation of ALA and BSA in freezing extender enhanced the sperm viability, mitochondrial intact and decrease acrosomal membrane damage. In conclusion, our findings suggest that quality of frozen-thawed sperm in mammalians could improve by using of ALA and BSA.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Semen Collection
  Semen Freezing and Thawing Process
  Analysis of Viability, Acrosome Reaction, and Mitochondrial Intact
  Statistical Analysis
 RESULTS
 DISCUSSION
 REFERENCES

키워드

Alpha-linolenic acid Bovine serum albumin Cryopreservation Sperm characteristics Pigs

저자

  • Won-Hee Lee [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea ]
  • Yong Hwangbo [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea ]
  • Sang-Hee Lee [ Institute of Animal Resources, Kangwon National University, Chuncheon 24341, Republic of Korea ]
  • Hee-Tae Cheong [ Hee-Tae Cheong3 | College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea ]
  • Boo-Keun Yang [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea ]
  • Choon-Keun Park [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 40 No 4

    피인용수 : 0(자료제공 : 네이버학술정보)

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