Earticle

초록

영어

Intracerebral hemorrhage (ICH) is a severe type of stroke causing neu-rological dysfunction with high mortality rate. Depression is one of the most common complications of ICH. In the present study, the effects of treadmill exercise on ICH-induced depressive symptoms in relation with apoptosis were investigated using rats. ICH rat model was induced by injection of collagenase into the hippocampus using stereotaxic instru-ment. Open field test for activity and forced swimming test for depres-sive symptoms were conducted. Apoptosis in the hippocampus was detected using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay, immunohistochemistry for caspase-3, and western blot for Bcl-2 and Bax. Western blot analysis for 5-hydroxy-tryptamine (5-HT, serotonin) and tryptophan hydroxylase (TPH) in the dorsal raphe was also conducted for biomarkers of depression. In the present results, immobility time was increased and climbing time was decreased by induction of ICH and treadmill exercise inhibited immobil-ity time and increased climbing time in ICH rats. DNA fragmentation and caspase-3 expression in the hippocampal dentate gyrus were en-hanced by induction of ICH and treadmill exercise suppressed ICH-in-duced DNA fragmentation and caspase-3 expression. Bax expression in the hippocampus was increased by induction of ICH and treadmill exercise inhibited Bax expression in the ICH rats. Expressions of 5-HT and TPH in the dorsal raphe were decreased by induction of ICH and treadmill exercise increased expressions of 5-HT and TPH in the ICH rats. In the present study, treadmill exercise ameliorated depressive symptoms through inhibiting apoptosis.

목차

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Experimental animals and treatments
  Induction of collagenase-induced ICH
  Exercise protocol
  Open field test
  Forced swimming test
  Preparation of tissues
  TUNEL staining
  Immunohistochemistry for caspase-3
  Immunohistochemistry for 5-HT and TPH
  Western blot analysis
  Data analysis
 RESULTS
 DISCUSSION
 CONFLICT OF INTEREST
 ACKNOWLEDGMENTS
 REFERENCES

키워드

저자

• Joo Hwan Roh [ Department of Urology, Gachon University Gil Medical Center, Gachon University School of Medicine, Incheon, Korea ]
• Il-Gyu Ko [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Sung-Eun Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Jae-Min Lee [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Eun-Sang Ji [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Ju Ho Kim [ Department of Urology, Gachon University Gil Medical Center, Gachon University School of Medicine, Incheon, Korea ]
• Hyun-Kyung Chang [ Department of Urology, Gachon University Gil Medical Center, Gachon University School of Medicine, Incheon, Korea ]
• Seung Kyu Lee [ Department of Urology, Gachon University Gil Medical Center, Gachon University School of Medicine, Incheon, Korea ]
• Khae Hawn Kim [ Department of Urology, Gachon University Gil Medical Center, Gachon University School of Medicine, Incheon, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613