Earticle

초록

영어

Cerebral ischemia is caused by reduced cerebral blood flow due to a transient or permanent cerebral artery occlusion. Ischemic injury in the brain leads to neuronal cell death, and eventually causes neurological impairments. Cordyceps, the name given to the fungi on insects, has abundant useful natural products with various biological activities. Cordyceps is known to have nephroprotective, hepatoprotective, an-ti-inflammatory, antioxidative, and antiapoptotic effects. We investigat-ed the effects of Cordyceps on short-term memory, neuronal apoptosis, and cell proliferation in the hippocampal dentate gyrus following tran-sient global ischemia in gerbils. For this study, a step-down avoidance test, terminal deoxynucleotidyl transferase-mediated dUTP nick end label-ing assay, immunohistochemistry for caspase-3 and 5-bromo-2’-de-oxyu-ridine, and western blot for Bax, Bcl-2, brain-derived neurotrophic fac-tor (BDNF), and tyrosin kinase B were performed. In the present study, Cordyceps alleviated cerebral ischemia-induced short-term memory impairment. Cordyceps showed therapeutic effects through inhibiting cerebral ischemia- induced apoptosis in the hippocampus. Cordyceps suppressed cerebral ischemia-induced cell proliferation in the hippo-campal dentate gyrus due to the reduced apoptotic neuronal cell death. Cordyceps treatment also enhanced BDNF and TrkB expressions in the hippocampus of ischemic gerbils. It can be suggested that Cordyceps overcomes cerebral ischemia-induced neuronal apoptosis, thus facili-tates recovery following cerebral ischemia injury.

목차

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Preparation of aqueous extract of Cordyceps
  Animals and treatments
  Induction of transient global ischemia
  Step-down avoidance test
  Tissue preparation
  TUNEL staining
  Immunohistochemistry for BrdU
  Western blotting
  Data analysis
 RESULTS
  Effect of Cordyceps on short-term memory in the step-down avoidance test
  Effect of Cordyceps on TUNEL-positive cells in the hippocampal CA1 region
  Effect of Cordyceps on caspase-3 expression in the hippocampal CA1 region
  Effect of Cordyceps on cell proliferation in the hippocampal dentate gyrus
  Effects of Cordyceps on Bax and Bcl-2 expressions in the hippocampus
  Effects of Cordyceps on BDNF and TrkB expressions in the hippocampus
 DISCUSSION
 CONFLICT OF INTEREST
 ACKNOWLEDGMENTS
 REFERENCES

키워드

저자

• Sang-Hak Lee [ Department of Life Science, College of Science & Engineering, Cheongju University, Cheongju, Korea ]
• Il-Gyu Ko [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Sung-Eun Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Lakkyong Hwang [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Jun-Jang Jin [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Hyun-Hee Choi [ Division of Leisure & Sports Science, Department of Exercise Prescription, Dongseo University, Busan, Korea ]
• Chang-Ju Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613