Earticle

초록

영어

Heavy industry has played pivotal role for the industry development in Korea forthe last half century and the facility requires periodic maintenance to keep consistency and stability because of harsh operating condition. However this might be carried out under oxygen depleted confined space. During the overhaul, suffocate might happen and the cause should be examined whether hypoxia is really involved. It is reported that hypoxia deaminates cytosine in DNA to uracil and then the DNA recovery system is known to be initiated. In this study, to get the information of uracil in the blood, SD (sprauge dawley) rats were exposed to the oxygen depleted confined space filled with nitrogen gas and then the blood was sampled followed by analyzed with GC-MS (gas chromatography-massspectrometry) through solvent extraction and silylation via standard addition method. The detection limit of the method for uracil was 0.01 μg/mL in water and the linearity of r2 showed better than 0.95. For all of the 7 blood samples, the concentration of uracil was from the value of less than 1.3 μg/mL for normal blood and 3.7 μg/mL through 10.1 μg/mL for the blood exposed to hypoxia condition. The concentration of uracil was found to be increased by about 9.6 times on average for 7 pairs. Additionally for the 3 cases of death assumed to be due to hypoxia in the confined space, the concentration of uracil was 21.8 μg/mL, 16.5 μg/mL and 10.0 μg/mL respectively. More sophisticated experiment should be required to confirm uracil to be used as a marker for the case of death in the oxygen depleted space because other variable could affect the concentration of uracil.

목차

Abstract
 I. 서론
 II. 실험
  1. 실험동물 및 시약
  2. 질식조건 설정 및 실험장비
  3. 실험방법
 III. 결과 및 고찰
  1. 유도체화 및 GC/MS 분석
  2. 실험동물 생체시료 중 uracil 농도
  3. 질식 사망사례 중 uracil 농도
 IV. 결론
 V. 참고문헌

키워드

저자

• 이준배 [ Joon-Bae Lee | 국립과학수사연구원 대구과학수사연구소 ] Corresponding author
• 손성건 [ Sung-Kun Shon | 대전보건대학교 과학수사과 ]
• 김춘삼 [ Choon-Sam Kim | 국립과학수사연구원 서울과학수사연구소 ]
• 박세연 [ Se-Yeon Park | 국립과학수사연구원 대구과학수사연구소 ]
• 황정호 [ Jung-Ho Hwang | 국립과학수사연구원 대구과학수사연구소 ]
• 우상희 [ Sang-Hee Woo | 국립과학수사연구원 대구과학수사연구소 ]
• 허상철 [ Sang-cheol Heo | 국립과학수사연구원 서울과학수사연구소 ]
• 김기욱 [ Ki-Wook Kim | 국립과학수사연구원 원주과학수사연구소 ]
• 권오성 [ O-Seong Kwon | 국립과학수사연구원 대구과학수사연구소 ]
• 유재훈 [ Jae-Hoon You | 국립과학수사연구원 서울과학수사연구소 ]
• 민지숙 [ Ji-Sook Min | 국립과학수사연구원 대구과학수사연구소 ]
• 팽기정 [ Ki-Jung Paeng | 연세대학교 화학과 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국법과학회지 [Korean Journal of Forensic Science]

• 간기

  반년간

• pISSN

  1598-0715

• 수록기간

  2000~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 367 DDC 347