Earticle

현재 위치 Home

Research Article

Isolation and Characterization of Mannanase-Producing Bacillus amyloliquefaciens YJ17 from Spent Mushroom (Flammulina velutipes) Substrates

첫 페이지 보기
  • 발행기관
    한국버섯학회 바로가기
  • 간행물
    한국버섯학회지 바로가기
  • 통권
    제14권 제1호 (2016.03)바로가기
  • 페이지
    pp.21-26
  • 저자
    Hye Soo Kim, Chul Hwan Kim, Hyun Sook Kwon, Soo Jeong Cho
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A270577

※ 기관로그인 시 무료 이용이 가능합니다.

4,000원

원문정보

초록

영어
The mannanase-producing bacteria, designated YJ17, was isolated from spent mushroom (Flammulina velutipes) substrates. The isolate YJ17 was a facultative anaerobic and was grown at temperatures ranging from 20oC to 50oC with an optimal temperature of 40oC. The DNA G+C content of the YJ17 was 44 mol%. The major fatty acids were anteiso-15:0 (38.9%), 17:0 (7.6%), and iso-15:0 (36.5%). The 16S rRNA gene sequence similarity between the isolate YJ17 and other Bacillus strains was from 98% to 99%. In the phylogenetic analysis based on these sequences, the isolate YJ17 and Bacillus amyloliquefaciens clustered within a group together and separated from other species of Bacillus. Based on the physiological and molecular properties, the isolate YJ17 was classified within the genus Bacillus as B. amyloliquefaciens YJ17. The optimal pH and temperature for mannanase activity of B. amyloliquefaciens YJ17 were pH 7.0 and 50oC, respectively.

목차

ABSTRACT
 Introduction
 Materials and Methods
  Isolation of mannanase-producing bacteria from spent mushroom substrates
  Morphological characteristics
  Fatty acid methyl ester analysis
  Utilization of carbon source
  DNA base composition
  Sequencing of 16S rRNA gene
  Sequence comparison and phylogenetic analysis
  Enzyme assays
 Results and Discussion
  Screening of mannan-degrading bacteria from from spent mushroom substrates
  Morphological and physiological characteristics
  Chemotaxonomy
  16S rRNA sequence analysis
  Effect of temperature and pH on mannanase activity
 References

키워드

Bacillus amyloliquefaciens YJ17 Mannanase-producing bacteria Spent mushroom substrates

저자

  • Hye Soo Kim [ Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea ]
  • Chul Hwan Kim [ Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea ]
  • Hyun Sook Kwon [ Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan, 712-260, Korea ]
  • Soo Jeong Cho [ Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국버섯학회 [The Korean Society of Mushroom Science]
  • 설립연도
    1997
  • 분야
    농수해양>농학
  • 소개
    우리나라의 버섯 연구와 관련 산업의 발전에 기여하고 버섯관련 연구자와 관련산업 종사자간의 학술정보 등의 교류를 목적으로 한다.

간행물

  • 간행물명
    한국버섯학회지 [Journal of MUSHROOMS]
  • 간기
    계간
  • pISSN
    1738-0294
  • eISSN
    2288-8853
  • 수록기간
    2003~2025
  • 등재여부
    KCI 등재
  • 십진분류
    KDC 525 DDC 635

이 권호 내 다른 논문 / 한국버섯학회지 제14권 제1호

    피인용수 : 0(자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      페이지 저장