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Developmental Potential of Bovine Nuclear Transfer Embryos Cultured in Serum-free Medium

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 27 No 4 (2003.12)바로가기
  • 페이지
    pp.339-347
  • 저자
    Lee, S. K, D. H. Kim, G. S. Im, B. C. Yang, H. S. Park, W. K. Chang, H. T. Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A26979

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초록

영어
The purpose of this study was to investigate the development of bovine nuclear transfer (NT) embryos cultured in serum-free conditions. Bovine NT embryos cultured in various culture conditions were compared blastocyst development, total cell number and apoptosis using TUNEL assay. In experiment 1, blastocyst rates of NT embryos were significantly higher (P<0.01) in FBS (22.0%) and BSA (26.6%) groups than in PVA (6.3%) group. Total cell number was significantly higher in FBS (78.4±19.4) and BSA (90.9±29.1) groups than in PVA group (46.0±0.0). Apoptotic cell number was significantly fewer in FBS (3.1±1.4) and BSA (1.7±1.4) groups than in PVA group (7.0±20.0) However, all of results were not different between the FBS and BSA group. In experiment 2, blastocyst rates of NT embryos were significantly higher (P<0.05) in fatty acid free-BSA (FAF-BSA) group (26.8%) than in fraction V-BSA group (11.2%). Total cell number were somewhat higher in FAF-BSA group (89.8±30.7) than in fraction V-BSA group (88.1±19.3). Apoptotic cell number were somewhat fewer in FAF-BSA (1.7±1.5) group than in fraction V-BSA group (4.2±2.9). These findings suggest that serum free condition were effective for the in vitro development of bovine NT embryos. Therefore, we concluded that fatty acid free-BSA has beneficial effect in development bovine NT embryos and can be use as a serum substitute.

키워드

Nuclear transfer FBS BSA PVA Apoptosis

저자

  • Lee, S. K [ Animal Biotechnology Division, National Livestock Research Institute. RDA., Animal Resources Research Center, Konkuk University ]
  • D. H. Kim [ Animal Biotechnology Division, National Livestock Research Institute. RDA. ]
  • G. S. Im [ Animal Biotechnology Division, National Livestock Research Institute. RDA. ]
  • B. C. Yang [ Animal Biotechnology Division, National Livestock Research Institute. RDA. ]
  • H. S. Park [ Animal Biotechnology Division, National Livestock Research Institute. RDA. ]
  • W. K. Chang [ Animal Biotechnology Division, National Livestock Research Institute. RDA. ]
  • H. T. Lee [ Animal Resources Research Center, Konkuk University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 27 No 4

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