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Global DNA Methylation of Porcine Embryos during Preimplantation Development

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 27 No 4 (2003.12)바로가기
  • 페이지
    pp.309-315
  • 저자
    Yeo S. E, Y. K. Kang, D. B. Koo, J. S. Han, K. Yu, C. H. Kim, H. Park, W. K. Chang, K. K. Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A26975

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원문정보

초록

영어
DNA methylation at CpG sites, which is a epigenetic modification, is associated with gene expression without change of DNA sequences. During early mouse embryogenesis, dynamic changes of DNA methylation occur. In this study, DNA methylation patterns of porcine embryos produced in vivo and in vitro were examined at various developmental stages by the immunocytochemical staining method. Interestingly, active demethylation was not observed on the paternal pronucleus of porcine zygotes. However, differences were detected in the passive demethylation process between in vivo and in vitro embryos. There was no change in the DNA methylation state until the blastocyst stage of in vivo embryos, whereas partial demethylation was observed in several blastomeres from a 4 cell stage to a morula stage of in vitro embryos. The whole genome of inner cell mass (ICM) and trophectoderm (TE) cells in porcine blastocysts were evenly methylated without de novo methylation.
Our findings demonstrate that genome-wide demethylation does not occur in pig embryos during preimplantation development unlike murine and bovine embryos. It indicates that the machinery regulating epigenetic reprogramming may be different between species.

키워드

DNA methylation 5-Methyl-cytosine Immunostaining Porcine embryos

저자

  • Yeo S. E [ Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology(KRIBB), Department of Biology, Chungnam National University ]
  • Y. K. Kang [ Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology(KRIBB) ]
  • D. B. Koo [ Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology(KRIBB) ]
  • J. S. Han [ Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology(KRIBB) ]
  • K. Yu [ Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology(KRIBB) ]
  • C. H. Kim [ Department of Biology, Chungnam National University ]
  • H. Park [ Department of Biotechnology, Taegu University ]
  • W. K. Chang [ National Livestock Research Institute ]
  • K. K. Lee [ Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology(KRIBB) ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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