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Genome-wide identification and characterization of novel laccase genes of Flammulina velutipes

첫 페이지 보기
  • 발행기관
    한국버섯학회 바로가기
  • 간행물
    버섯 바로가기
  • 통권
    제19권 2호 통권 32호 (2015.11)바로가기
  • 페이지
    pp.87-87
  • 저자
    Hong-Il Kim, O-Chul Kwon, Won-Sik Kong, Chang-Soo Lee, Young-Jin Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A268709

원문정보

초록

영어
The aim of this study was to identify and characterize new Flammulina velutipes laccases from its whole-genome sequence. Of the 15 putative laccase genes detected in the F. velutipes genome, four new laccase genes (fvLac-1, fvLac-2, fvLac3, and fvLac-4) were found to contain four complete copper-binding regions (ten histidine residues and one cysteine residue) and four cysteine residues involved in forming disulfide bridges. fvLac-1, fvLac-2, fvLac3, and fvLac-4, encoding proteins consisting of 516, 518, 515, and 533 amino acid residues, respectively. Potential N-glycosylationsites(Asn-Xaa-Ser/Thr) were identified in the cDNA sequence of fvLac-1 (Asn-454), fvLac-2(Asn- 437andAsn-455), fvLac-3(Asn-111andAsn-237), and fvLac4 (Asn-402andAsn-457). In addition, the first 19–20 amino acid residues of these proteins were predicted to comprise signal peptides. Laccase activity assays and reverse transcription polymerase chain reaction(RT-PCR) analyses clearly reveal that CuSO4 affects the induction and the transcription level of these laccase genes.

저자

  • Hong-Il Kim [ Department of Biomedical Chemistry, Konkuk University, Chungju, Korea ]
  • O-Chul Kwon [ Department of Biomedical Chemistry, Konkuk University, Chungju, Korea ]
  • Won-Sik Kong [ Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Korea ]
  • Chang-Soo Lee [ Department of Biomedical Chemistry, Konkuk University, Chungju, Korea ]
  • Young-Jin Park [ Department of Biomedical Chemistry, Konkuk University, Chungju, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국버섯학회 [The Korean Society of Mushroom Science]
  • 설립연도
    1997
  • 분야
    농수해양>농학
  • 소개
    우리나라의 버섯 연구와 관련 산업의 발전에 기여하고 버섯관련 연구자와 관련산업 종사자간의 학술정보 등의 교류를 목적으로 한다.

간행물

  • 간행물명
    버섯 [Mushroom]
  • 간기
    반년간
  • 수록기간
    1997~2025
  • 십진분류
    KDC 525 DDC 635

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