Earticle

현재 위치 Home

Poster Session I : 품종육성, 유전생리, 유전체

Differentiation of Pleurotus eryngii Cultivars by Multiplex Microsatellite Marker using Simple Sequence Repeats (SSR)

첫 페이지 보기
  • 발행기관
    한국버섯학회 바로가기
  • 간행물
    버섯 바로가기
  • 통권
    제19권 2호 통권 32호 (2015.11)바로가기
  • 페이지
    pp.86-86
  • 저자
    Asjad Ali, Chak Han Im, Bokyung Park, Min Keun Kim, Wan-Kyu Joung, Yong Jo Choi, Jae-San Ryu
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A268707

원문정보

초록

영어
Differentiation of Pleurotus eryngii is laborious and time-consuming tasks especially in mycelial status. For development of a method for differentiation of P. eryngii cultivars, simple sequence repeats (SSR) from whole genomic DNA sequence analysis was used for genotyping and two multiplex-SSR primer sets were developed. These SSR primer sets were employed to distinguish 12 cultivars and strains. Five polymorphic markers were selected based on the genotypes. PCR with the each primer produced one to four distinct bands ranging in size from 200 to 300 bp. Polymorphism information content (PIC) values of the five markers were in range of 0.6627 to 0.6848 with an average of 0.6775. Unweighted pair-group method with arithmetic mean clustering analysis based on genetic distances using five SSR markers classified 12 cultivars into 2 clusters. Cluster I and II comprised of 4 and 8 cultivars, respectively. Two multiplex sets, Multi-1 (SSR312 and SSR366) and Multi-2 (SSR178 and SSR277) completely discriminated 12 cultivar and strains with 21 allele with a PIC value of 0.9090. These results might be useful to provide an efficient method for the identification of P. eryngii cultivars with separate PCR reactions. (This work was supported by a grant from the Gold Seed Project [Supported by a grant from the IPET (213003-04-3-WTI11), MIFAFF, Republic of Korea.]

저자

  • Asjad Ali [ Environment-friendly research division, Gyeongsangnam-do Agricultural Research and Extension Services, Jinju 660-360, Korea ]
  • Chak Han Im [ Environment-friendly research division, Gyeongsangnam-do Agricultural Research and Extension Services, Jinju 660-360, Korea ]
  • Bokyung Park [ Environment-friendly research division, Gyeongsangnam-do Agricultural Research and Extension Services, Jinju 660-360, Korea ]
  • Min Keun Kim [ Environment-friendly research division, Gyeongsangnam-do Agricultural Research and Extension Services, Jinju 660-360, Korea ]
  • Wan-Kyu Joung [ Environment-friendly research division, Gyeongsangnam-do Agricultural Research and Extension Services, Jinju 660-360, Korea ]
  • Yong Jo Choi [ Environment-friendly research division, Gyeongsangnam-do Agricultural Research and Extension Services, Jinju 660-360, Korea ]
  • Jae-San Ryu [ Environment-friendly research division, Gyeongsangnam-do Agricultural Research and Extension Services, Jinju 660-360, Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국버섯학회 [The Korean Society of Mushroom Science]
  • 설립연도
    1997
  • 분야
    농수해양>농학
  • 소개
    우리나라의 버섯 연구와 관련 산업의 발전에 기여하고 버섯관련 연구자와 관련산업 종사자간의 학술정보 등의 교류를 목적으로 한다.

간행물

  • 간행물명
    버섯 [Mushroom]
  • 간기
    반년간
  • 수록기간
    1997~2025
  • 십진분류
    KDC 525 DDC 635

이 권호 내 다른 논문 / 버섯 제19권 2호 통권 32호

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.