Earticle

초록

영어

Hexane, ethyl acetate and methanol extracts of whole plant of Boerhavia diffusa were screened for phytochemical and biological activities. Qualitative phytochemical screening via colorimetric method and the quantitative estimation of phenolic and flavonoid content were performed. Antioxidant assay using DPPH scavenging method was studied. Antimicrobial screening of plant extracts was done by cup diffusion technique. Cytotoxic activity of B. diffusa was studied by brine shrimp bioassay and anthelminthic activity was evaluated in vitro in Pheretima posthuma. This study revealed B. diffusa as a source of various phyto-constituents such as alkaloids, glycosides, saponins, tannins, carbohydrates, cardiac glycosides, flavonoids and terpenoids. Quantitative estimation of total phenol was found to be maximum in BEE i.e. 29.73±0.88, BME 19.8±2.02 and in BHE 9.15±0.304 mgGAE/g. Similarly, the total flavonoid content was found to be 17.44±0.75 in BEE, 14.43±0.23 in BHE and 3.678 mg QE/g in BME. Ethyl acetate extract showed its antibacterial activity against all tested pathogens except Escherichia coli whereas Staphylococcus aureus and Salmonella Typhi were resistant to methanol and hexane extract. The zone of inhibition (ZOI) of ethyl acetate extract against S. Typhi and B. cereus was found to be 18 mm and 14 mm respectively. The MIC value of BEE in S. Typhi was 3.125 μg/ml and in B. cereus was 12.5 μg/ml. The preliminary screening of anticancer property of B. diffusa i.e. BSLT in methanol was found to be 165.19 μg/ml. B. diffusa was also found to contain anthelmintic property. The study helped in further exploration of medicinal properties of B. diffusa by phytochemical screening and biological activities paving the path for study and investigation in this plant.

목차

Abstract
 1. Introduction
 2. Materials and Methods
  2.1. Sample Collection and Extraction
  2.2. Test Animals and Pathogens
  2.3. Chemicals, Reagents and Standard Drugs
  2.4. Phytochemical Screening
  2.5. Biological Studies
  2.6. Antioxidant Activity Test
  2.7. Minimum Inhibitory Concentration (MIC) Determination
  2.8. Brine Shrimp Bioassay
  2.9. Anti – helminthic Activity
 3. Results and Discussion
  3.1. Qualitative Phytochemical Screening
  3.2. Total Polyphenol and Flavonoid Content Determination
  3.3. Antioxidant Activity Test
  3.4 Antimicrobial Activity and MIC Determination
  3.5. Brine Shrimp Bioassay
  3.6. Evaluation of Anti-helminthic Activity
 4. Conclusion
 References

키워드

저자

• Prakriti Gautam [ Department of Pharmacy, National Model College for Advance Learning, Tribhuvan University, Kathmandu, Nepal, Both authors contributed equally to this work. ]
• Sandesh Panthi [ Department of Pharmacy, Chosun University, Gwangju, South Korea, Both authors contributed equally to this work ]
• Prashubha Bhandari [ Department of Microbiology, National College, Tribhuvan University, Kathmandu, Nepal ]
• Jihoon Shin [ Department of Complementary & Alternative Medicine, Chosun University, Gwangju 501-759, Korea ]
• Jin Cheol Yoo [ Department of Pharmacy, Chosun University, Gwangju, South Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  통합자연과학논문집(구 조선자연과학논문집) [Journal of Integrative Natural Science]

• 간기

  계간

• pISSN

  2005-1042

• 수록기간

  2008~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 405 DDC 505