Earticle

초록

영어

Most of the bacterial and other simple non glycosylated recombinant proteins were conventionally produced from IPTG inducible Escherichia coli BL21(DE3). Considering the factors like cost and toxic nature of IPTG, as an alternative, salt inducible Escherichia coli GJ1158 was used in this study for the over production of staphylokinase variant (sak – hirulog) using fed batch fermentation, cost effectively. Optimization of physico chemical factors viz., dissolved oxygen (DO), carbon, nitrogen and phosphate sources on bacterial growth for the production of recombinant sak – hirulog using batch and fed batch fermentation was studied. In batch culture, increased DO at more than 30 % did not influence the enhanced expression of sak – hirulog, but significant improvement was observed in fed batch cultivation. Supplementation of production medium with different nutrient sources like dextrose, yeast extract and dipotassium hydrogen phosphate (K2HPO4) enhanced the sak – hirulog expression in fed batch fermentation process even without disturbing the cell growth by providing 50 % DO. Approximately 1178 mg/L of specific protein was obtained using cost effective modified glucose yeast exgtract (GYE) media devoid of sodium chloride. This study also reports the highest concentration of recombinant protein from salt inducible expression host till to date, which manages to satisfy the production of bifunctional staphaphylokinase variant using economically feasible bacterial expression host Escherichia coli GJ1158.

목차

Abstract
 1. Introduction
 2. Materials and Methods
  2.1. Strains and Plasmid
  2.2. Medium Preparation
  2.3. Inoculum Preparation
  2.4. Shake Flask Cultivation
  2.5. Fermentation
  2.6. Analytical Methods
  2.7. Fibrinolytic and Thrombolytic Activity
  2.8. Purification of Sak - Hirulog
 3. Results and Discussion
  3.1. Shake Flask Cultivation
  3.2. Batch Cultivation
  3.3. Fed batch Cultivation
  3.4. Effect of Dextrose
  3.5. Effect of Dissolved Oxygen
  3.6. Effect of Yeast Extract
  3.7. Effect of Disodium Hydrogen Phosphate
 4. Conclusion
 References

키워드

저자

• K. Seetha Ram [ Department of Biotechnology, Acharya Nagarjuna University, Guntur – 522510, India ]
• K. Satish Babu [ Department of Biochemistry, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India ] Co first authors
• G. Tabitha [ Center for Biotechnology, JNTU-H, Telangana, India ] Co first authors
• K. Rajeshwari [ Center for Biotechnology, JNTU-H, Telangana, India ] Co first authors
• G. Jaya Lakshmi [ Department of Biotechnology, Acharya Nagarjuna University, Guntur – 522510, India ]
• B. Boje Gowd [ Center for Biotechnology, JNTU-H, Telangana, India ]
• J. B. Peravali [ Department of Biotechnology, Acharya Nagarjuna University, Guntur – 522510, India ]
• M. Subba Rao [ Department of Biotechnology, Acharya Nagarjuna University, Guntur – 522510, India ]
• P. Venugopala Rao [ Department of Biotechnology, Acharya Nagarjuna University, Guntur – 522510, India ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  International Journal of Bio-Science and Bio-Technology

• 간기

  격월간

• pISSN

  2233-7849

• 수록기간

  2009~2016

• 등재여부

  SCOPUS

• 십진분류

  KDC 505 DDC 605