Earticle

초록

영어

To overcome the hyperacute immune rejection during pig-to-non-human primates xenotranasplantation, we have produced and bred α-1,3-galactosyltransferase knock-out (GalT —/—) pigs. In this study, the somatic cells and tissues from the GalT —/— pigs were characterized by an analysis of the expression of Galα-1,3-Gal (α- Gal) epitope. Briefly, ear fibroblast cell lines of 19 homozygous GalT —/— pigs were established and cryopreserved. The expression of α-Gal epitope in the cells was measured by fluorescence activated cell sorter (FACS) analysis using BS- I-B4 lectin. Also, the homozygous (GalT —/—) cells and tissues samples were immunostained with BS-I-B4 lectin for analysis of α-Gal epitope expression. The results showed that the expression of α-Gal epitope in GalT —/— cells (0.2 %) were significantly (p< 0.05) down-regulated to the range of cynomolgus monkey fibroblast (0.2 %) cells compared to heterozygous (GalT —/+) (9.3 %) and wild type (GalT +/+) (93.7 %) fibroblast cells. In the immunostaining results, while the expression of α-Gal epitope was detected a partly in GalT —/+ cells and mostly in GalT +/+ cells, it was almost not detected in the GalT —/— cells. Also, immunostaining results from various tissues of the GalT —/— pig showed that the expression of α-Gal epitope was not detectable, whereas various tissues from GalT +/+ pig showed a strong expression of α-Gal epitope. Our results demonstrated that α-Gal epitope expressions from GalT —/— pigs were successfully knocked out to prevent hyperacute immune rejection for further study of xenotransplantation.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  General Information
  Establishment of Ear Fibroblast Cell Lines
  Fluorescence Activated Cell Sorter Analysis
  Immunostaining of Cells and Tissues
  Statistics
 RESULTS
  α-Gal Epitope Expressions by FACS Analysis
  α-Gal Epitope Expressions by Immunostaining
 DISCUSSION
 REFERENCES

키워드

저자

• In-Sul Hwang [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Jeonbuk 55365, Republic of Korea ]
• Dae-Jin Kwon [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Jeonbuk 55365, Republic of Korea ]
• Tae-Uk Kwak [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Jeonbuk 55365, Republic of Korea ]
• Keon Bong Oh [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Jeonbuk 55365, Republic of Korea ]
• Sun-A Ock [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Jeonbuk 55365, Republic of Korea ]
• Hak-Jae Chung [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Jeonbuk 55365, Republic of Korea ]
• Gi-Sun Im [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Jeonbuk 55365, Republic of Korea ]
• Seongsoo Hwang [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Jeonbuk 55365, Republic of Korea ] Corresponding author

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간행물

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• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636