Earticle

초록

영어

Iron is required for cell viability but is toxic in excess. While the iron-mediated malfunction of testicular cells is well appreciated, the underlying mechanism(s) of this effect and its relationship with fertility are poorly understood. Ferritin is a ubiquitous intracellular protein that controls iron storage, ferroxidase activity, immune response, and stress response in cells. Ferritin light chain protein (FTL) is the light subunit of the Ferritin. Previously, we had identified the FTL in bovine spermatozoa following capacitation. In present study, to investigate the role of Ferritin in sperm function, mice spermatozoa were incubated with multiple doses (1, 10 and 100 μM) of sodium nitroprusside (SNP), an iron donor. SNP was increased Ferritin levels in a dose-dependent manner. The Ferritin was detected on the acrosome in spermatozoa by immunocytochemistry. Short-term exposure of spermatozoa to SNP increased tyrosine phosphorylation and the acrosome reaction (AR). Finally, SNP affected a significant decrease in the rate of fertilization as well as blastocyst formation during early embryonic development. On the basis of these results, we propose that the effects of Ferritin on the AR may reduce overall sperm function leads to poor fertility in males and compromised embryonic development.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND MATHODS
  Ethical Statement
  Media and Chemicals
  Preparation and Treatment of Mouse Spermatozoa
  Western Blot Analysis for Detection of Sperm Proteins
  Combined Hoechst 33258/chlortetracycline FluorescenceAssessment of Capacitation Status (H33258/CTC)
  Localization of Ferritin in Spermatozoa
  In Vitro Fertilization
  Statistical Analysis
 RESULTS
  Effect of SNP on Ferritin Levels in Spermatozoa
  Effect of SNP on Spermatozoa Capacitation Status
  Effect of SNP on Spermatozoa Phosphotyrosine Levels
  Effect of SNP on Fertilization and Embryo Development
 DISCUSSION
 REFERENCES

키워드

저자

• Woo-Sung Kwon [ Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Korea ]
• Md Saidur Rahman [ Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Korea ]
• Ye-Ji Kim [ Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Korea ]
• Do-Yeol Ryu [ Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Korea ]
• Amena Kahtun [ Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Korea ]
• Myung-Geol Pang [ Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636