Earticle

초록

영어

Galactose-α1,3-galactose (α1,3-Gal) epitope is synthesized at a high concentration on the surface of pig cells by α1,3-galactosyltransferase gene (GGTA1). The α1,3-Gal is responsible for hyperacute rejection in pig-to-human xenotransplantation. The generation of transgenic pigs as organ donors for humans is necessary to eliminate the GGTA1 gene that synthesize Galα(1,3)Gal. To prevent hyperacute graft rejection in pig-to-human xenotransplantation, previously, we developed α1,3-galactosyltransferase gene-knock-out somatic cell by homologous recombination. In this study, we established cell lines of α1,3-GT knock-out expressing hDAF and hHT gene from minipig fibroblasts to apply somatic cell nuclear transfer. The hDAF and hHT mRNA were expressed in the knock-in somatic cells and α1,3-GT mRNA was suppressed. However, the knock-in somatic cells were increased resistance to human serummediated cytolysis.

목차

서 론
 재료 및 방법
  α1,3-Galactosyltransferase 유전자 위치에서 hDAF와 HT유전자가 발현하는 knock-in vector 제작
  미니돼지 체세포 배양
  미니돼지 체세포에 knock-in vector 도입과 세포 선별
  Knock-in된 체세포의 PCR 분석
  RT-PCR과 Real-time PCR
  Cytotoxicity assay
 결과 및 고찰
  α1,3-GT 유전자 위치에서 hDAF와 hHT 유전자가 발현하는 knock-in vector 구축
  α1,3GT-hDAF-hHT knock-in vector 도입 및 선별
  α1,3GT-hDAF-hHT knock-in 체세포에서 α1,3-GT, hDAF,hHT 유전자 발현 분석
  α1,3GT-hDAF-hHT knock-in 체세포에서 human complementserum 처리에 의한 cytotoxicity 분석
 요 약
 REFERENCES

키워드

저자

• 김지우 [ Ji Woo Kim | 전남대학교 농업생명과학대학 동물자원학부 ]
• 강만종 [ Man-Jong Kang | 전남대학교 농업생명과학대학 동물자원학부 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636