Earticle

초록

영어

In order to achieve successful in vitro production of embryo, it is necessary to establish intrauterine environment during in vitro culture. Thus, this study was investigated to establish embryo culture system using co-incubated collagen matrix gel (CM) with endometrial epithelial cells (EC). Endometrial epithelial cells were isolated from porcine endometrium at follicular phase, the cells seeded in insert dish for co-incubation with CM-coated culture dish. Then, culture media treated with/without 2.0 IU/ml hCG or 10 ng/ml IL-1β. After incubation for 24 h, the co-incubated insert dishes were removed from CM-coated culture dish before embryo culture. Embryos at 48 h after in vitro fertilization (IVF) were cultured on the dish for 120 h with porcine zygote medium. We determined PTGS-2 expression in the ECs, VEGF protein in co-incubated CM with EC and observed cleavage rate and blastocyst development of embryos at 168 h after IVF. In result, expression of PTGS-2 was higher at co-incubated EC with hCG and IL-1β groups than EC without hCG and IL-1β. The VEGF protein was detected at co-incubated CM with EC, EC treated with hCG and IL-1β groups higher than CM group. Also, cleavage rate was no significantly difference among all group, however, blastocyst development was significantly higher in co-incubated CM with EC treated with hCG group than un-treated groups (p<0.05). Therefore, we suggest that novel embryo culture system using co-incubated collagen matrix gel with endometrial epithelial cells treated with IL-1β is beneficial and useful for enhancing the production of porcine blastocysts in vitro.

목차

서 론
 재료 및 방법
  자궁내막세포의 분리 및 배양
  실험설계
  체외수정란 생산
  Conditioned collagen matrix 제작
  체외수정란 배양
  Quantitative RT-PCR
  통계처리
 결 과
  자궁내막 상피세포에서 황체기 시기 발현되는 유전물질 발현 확인
  자궁내막 상피세포와 공동배양된 CM에 결합된 VEGF 단백질 확인
  체외수정 능력 검
 고 찰
 감사의 글
 REFERENCES

키워드

저자

• 이상희 [ Sang-Hee Lee | 강원대학교 동물생명과학대학 ]
• 한혜인 [ Hye-In Han | 강원대학교 동물생명과학대학 ]
• 황보용 [ Yong Hwangbo | 강원대학교 동물생명과학대학 ]
• 이승형 [ Seunghyung Lee | 강원대학교 동물생명과학대학 ]
• 정희태 [ Hee-Tae Cheong | 강원대학교 수의과대학 ]
• 양부근 [ Boo-Keun Yang | 강원대학교 동물생명과학대학 ]
• 박춘근 [ Choon-Keun Park | 강원대학교 동물생명과학대학 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636