Earticle

초록

영어

Huntington’s disease is a chronic neurodegenerative disorder inherited in an autosomal dominant fashion, and characterized as involuntary movement. Quinolinic acid has been used to produce an animal model of Huntington’s disease. In the present study, the effect of treadmill ex-ercise on spatial-learning ability and motor coordination focusing on the apoptosis in the hippocampus was investigated using quinolinic ac-id-induced Huntington’s disease rats. Huntington’s disease was induced by unilateral intrastriatal injection of quinolinic acid (2 μL of 100 nmol) using stereotaxic instrument. The rats in the treadmill exercise groups were subjected to run on a treadmill for 30 min once a day during 14 days. Spatial learning ability and motor coordination were determined by radial 8-arm maze test and rota-rod test. Immunohistochemistry for caspase-3 and western blot for Bax and Bcl-2 were also conducted for the detection of apoptosis. In the present results, spatial learning ability and motor coordination were deteriorated by intrastriatal injection of quinolinic acid. In contrast, treadmill exercise exerted ameliorating ef-fect on quinolinic acid-induced deterioration of spatial learning ability and motor coordination. Bcl-2 expression in the hippocampus was de-creased and expressions of casepase-3 and Bax in the hippocampus were increased in the quinolinic acid-induced Huntington’s disease rats. Treadmill exercise increased Bcl-2 expression and decreased ex-pressions of casepase-3 and Bax in the Huntington’s disease rats. The present results showed that treadmill exercise might ameliorate quino-linic acid-induced loss of spatial learning ability and motor coordination by suppressing apoptosis in the hippocampus.

목차

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Animals
  Induction of Huntington’s disease model
  Treadmill exercise protocol
  Radial 8-arm maze test
  Tissue preparation
  Caspase-3 immunohistochemistry
  Western blot analysis of Bax and Bcl-2 expressions
  Data analysis
 RESULTS
  Effect of treadmill exercise on spatial learning ability in the radial 8-arm maze test
  Effect of treadmill exercise on the motor coordination in the rota-rod test
  Effect of treadmill exercise on the caspase-3 expression in the hippocampal dentate gyrus
  Effect of treadmill exercise on the expressions of Bax and Bcl-2 in the hippocampus
 DISCUSSION
 CONFLICT OF INTEREST
 ACKNOWLEDGMENTS
 REFERENCES

키워드

저자

• Eun-Sang Ji [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• You-Mi Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Mal-Soon Shin [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Chang-Ju Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Kwang-Sik Lee [ Research Institute of Sports Science, National University of Incheon, Incheon, Korea ]
• Kijeong Kim [ Department of Exercise & Sport Science, College of Natural Sciences, University of Ulsan, Ulsan, Korea ]
• Jonglin Ha [ Department of Anesthesiology and Pain Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea ]
• Yong-Rak Chung [ Department of Golf Mapping, College of Arts Physical Education, Joongbu University, Geumsan-gun, Chungcheongnam-do, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613