Earticle

초록

영어

Diabetes is a metabolic disorder, and it is considered as a major risk factor for Alzheimer’s disease (AD). In the present study, we evaluated whether treadmill exercise ameliorates progression of AD in relation with glycogen synthase kinase-3β (GSK-3β) activity using streptozoto-cin (STZ)-induced diabetic rats. For this study, step-down avoidance task, immunohistochemistry for glycogen synthase kinase-3β (GSK-3β) and tau, and western blot for phosphor-phosphoinositide 3 kinase (p-PI3K)/PI3K and phosphor-Akt (p-Akt)/Akt were performed. Diabetes mellitus was induced by intraperitoneal injection of STZ. The rats in the exercise groups were made to run on the treadmill for 30 min per one day, five times a week, during 12 weeks. The present results showed that short-term and long-term latencies in the step-down avoidance task were decreased by induction of diabetes, and treadmill exercise inhibited these latencies in the diabetic rats. Induction of diabetes sup-pressed the ratio of p-PI3K to PI3K and the ratio of p-Akt to Akt, and treadmill exercise increased these ratios in the diabetic rats. The num-bers of GSK-3β-positive and tau-positive cells in the hippocampal den-tate gyrus was higher in the diabetes-induction group than that in the control group, and treadmill exercise inhibited these numbers in the di-abetic rats. In the present study, treadmill exercise suppressed hyper-phosphorylation of tau in the hippocampus by decreased GSK-3β activ-ity through PI3K/Akt pathway activation in the diabetic rats. Based on the present results, treadmill exercise may helpful to prevent diabe-tes-associated AD occurrence.

목차

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Animals and treatment
  Induction of diabetes
  Exercise protocol
  Step-down avoidance task
  Tissue preparation
  Immunohistochemistry for GSK-3β and Tau
  Western blot analysis
  Data analysis
 RESULTS
  Effect of treadmill exercise on the latency in the step-downavoidance task
  Effect of exercise on phospho-PI3K (p-PI3K) and PI3K expressions in the hippocampus
  Effect of exercise on phospho-Akt (p-Akt) and Akt expressions in the hippocampus
  Effect of exercise on the number of GSK-3~-positive cells in the hippocampal dentate gyrus
  Effect of exercise on the number of tau-positive cells in the hippocampal dentate gyrus
 DISCUSSION
 CONFLICT OF INTEREST
 ACKNOWLEDGMENTS
 REFERENCES

키워드

저자

• Dae-Young Kim [ Department of Sports Healthcare, College of Humanities & Social Sciences, lnje University, Gimhae, Korea ]
• Sun-Young Jung [ Department of Physical Therapy, Hasan University, Gyeongsan, Korea ]
• Tae-Woon Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Kwang-Sik Lee [ Research Institute of Sports Science, National University of lncheon, lncheon, Korea ]
• Kijeong Kim [ Department of Exercise & Sport Science, College of Natural Sciences, University of Ulsan, Ulsan, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613